{"id":"c168afd5-ac55-58d0-99f8-3ee1ab7413dc","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:intraduodenal-nattokinase-degrades-plasma-fibrinogen-in-rat","predicate":"proteolyzes","statement":"Nattokinase injected into a tied intestinal loop at 80 mg/kg was followed by fibrinogen degradation products in plasma in all twenty rats, with a 270 kDa fragment appearing within 0.5 to 5 h.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"730ed911-98cf-5974-8a94-74089e6433f0","mechanism_event_label":"Nattokinase injected into a tied intestinal loop at 80 mg/kg was followed by fibrinogen degradation products in plasma in all twenty rats, with a 270 kDa fragment appearing within 0.5 to 5 h.","subject":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"object":{"id":"d0f26332-5d2c-509e-a255-344eec884b71","slug":"rat-fibrinogen","display_name":"Rat fibrinogen","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"730ed911-98cf-5974-8a94-74089e6433f0","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:intraduodenal-nattokinase-degrades-plasma-fibrinogen-in-rat-event","event_type":"observed_intervention","label":"Nattokinase injected into a tied intestinal loop at 80 mg/kg was followed by fibrinogen degradation products in plasma in all twenty rats, with a 270 kDa fragment appearing within 0.5 to 5 h.","description":"Ligated intestinal loop including duodenum and jejunum, femoral-vein plasma","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d0f26332-5d2c-509e-a255-344eec884b71","slug":"rat-fibrinogen","display_name":"Rat fibrinogen","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Sampling to 5 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat (male Wistar, n = 20)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Nattokinase 80 mg/kg","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The stomach was bypassed, so this cannot establish gastric survival after ordinary oral dosing, and there was no control group receiving the same surgery with placebo. Breakdown patterns differed between animals: fourteen of twenty showed a 270 kDa fragment at 3 to 5 h with no further degradation, while six showed it at 0.5 h followed by degradation to a 105 kDa fragment via a 200 kDa intermediate. The abstract narrates only the six-rat pattern; the fourteen-rat pattern is recorded in the EFSA account of the same study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rat (male Wistar, n = 20)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Nattokinase injected into a tied intestinal loop at 80 mg/kg was followed by fibrinogen degradation products in plasma in all twenty rats, with a 270 kDa fragment appearing within 0.5 to 5 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of nattokinase across the rat intestinal tract. 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Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. 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