{"id":"c1662d09-1e91-50f4-bf16-a2d754397b04","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-gcl","predicate":"increases_tested_expression_of","statement":"Ankaflavin increased hepatic GCL and HO-1 transcriptional activation; these effects persisted despite PPAR-gamma antagonist treatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a8b4f416-2930-5d2d-9323-0122be4d8a71","mechanism_event_label":"The antioxidant-expression route was not blocked in the same way as the insulin endpoints.","subject":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"object":{"id":"7a1372c6-d4d9-50e6-85bf-b07f345a886c","slug":"rat-gcl-pigment-study","display_name":"Rat hepatic glutamate-cysteine ligase expression in the ankaflavin study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a8b4f416-2930-5d2d-9323-0122be4d8a71","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-ankaflavin-gcl-event","event_type":"observed_relationship","label":"The antioxidant-expression route was not blocked in the same way as the insulin endpoints.","description":"Ankaflavin increased hepatic GCL and HO-1 transcriptional activation; these effects persisted despite PPAR-gamma antagonist treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"92939faa-84d1-52e8-a70f-deefd2486d52","slug":"ankaflavin","display_name":"Ankaflavin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a1372c6-d4d9-50e6-85bf-b07f345a886c","slug":"rat-gcl-pigment-study","display_name":"Rat hepatic glutamate-cysteine ligase expression in the ankaflavin study","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"27636f1d-fd51-5697-a8bb-6c827f4f8b40","slug":"rat-nfe2l2","display_name":"Rat Nrf2 / Nfe2l2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wistar rat methylglyoxal model with GW9662 comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"GCL subunit-specific effects were not resolved in the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Red yeast rice collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"red-yeast-rice","display_name":"Red yeast rice","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The antioxidant-expression route was not blocked in the same way as the insulin endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[23022408] Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23022408/ · DOI 10.1016/j.freeradbiomed.2012.09.025","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"01868f49-d76f-57c9-8c29-b7cdb68059fa","evidence_kind":"source_excerpt","locator":"Lines 252-258","start_line":252,"end_line":258,"excerpt":"## red-yeast-rice-ankaflavin-gcl\nThe antioxidant-expression route was not blocked in the same way as the insulin endpoints.\nAnkaflavin increased hepatic GCL and HO-1 transcriptional activation; these effects persisted despite PPAR-gamma antagonist treatment.\nModel: Wistar rat methylglyoxal model with GW9662 comparison.\nLimitations: GCL subunit-specific effects were not resolved in the accessed abstract.\nEvidence access: Primary abstract\n[23022408] Ankaflavin: a natural novel PPARγ agonist upregulates Nrf2 to attenuate methylglyoxal-induced diabetes in vivo. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23022408/ · DOI 10.1016/j.freeradbiomed.2012.09.025","model_system":"Wistar rat methylglyoxal model with GW9662 comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"261a12bc-3d00-52a4-a5ec-70e174390596","stable_key":"import-4910e17b-aaec-52cd-b9e5-caca85d817ee","title":"Red yeast rice: constituents, mevalonate, CoQ and product-specific interactions (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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