{"id":"c13bb3dc-56d2-5785-acd6-e6a4dfef3727","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-human-epigenetic-enzymes","predicate":"reduces","statement":"Luteolin reduced measured DNMT and HDAC protein levels and activities in HCT116 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"91de542d-4837-53a3-b938-ae0fd3119545","mechanism_event_label":"Several regulators changed together.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"6c973a47-2258-51d3-b0a0-b54c663e9ad4","slug":"human-hct116-dnmt-hdac-activity","display_name":"Human HCT116 DNMT/HDAC measured activity pools","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"91de542d-4837-53a3-b938-ae0fd3119545","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-human-epigenetic-enzymes-event","event_type":"observed_relationship","label":"Several regulators changed together.","description":"Luteolin reduced measured DNMT and HDAC protein levels and activities in HCT116 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6c973a47-2258-51d3-b0a0-b54c663e9ad4","slug":"human-hct116-dnmt-hdac-activity","display_name":"Human HCT116 DNMT/HDAC measured activity pools","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cultured cancer cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Readout does not prove direct binding to each enzyme or identify one causal isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Several regulators changed together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30129150/ · DOI 10.1002/jcb.27275","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb2d2989-0852-596a-9dac-5d5b8b96fd7c","evidence_kind":"source_excerpt","locator":"Lines 476-482","start_line":476,"end_line":482,"excerpt":"## luteolin-human-epigenetic-enzymes\nSeveral regulators changed together.\nLuteolin reduced measured DNMT and HDAC protein levels and activities in HCT116 cells.\nModel: Human cultured cancer cells.\nLimitations: Readout does not prove direct binding to each enzyme or identify one causal isoform.\nEvidence access: Primary abstract\nThe dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30129150/ · DOI 10.1002/jcb.27275","model_system":"Human cultured cancer cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}