{"id":"c123e041-dee4-52f9-a2c1-ae3b27555f88","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:cldn16-cldn19-cation-selectivity","predicate":"cooperates_with","statement":"Claudin-16/19 coexpression increases paracellular cation selectivity in LLC-PK1 epithelial monolayers.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"550a7d12-e102-50c7-8607-85f365329079","mechanism_event_label":"These renal junction proteins work together.","subject":{"id":"b9e564ce-7834-5815-a22b-0aa555a7bfe4","slug":"cldn16","display_name":"Claudin-16","entity_type_key":"protein"},"object":{"id":"2e41ca25-b273-5974-8e13-cfa2654da636","slug":"cldn19","display_name":"Claudin-19","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"550a7d12-e102-50c7-8607-85f365329079","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:cldn16-cldn19-cation-selectivity-event","event_type":"biochemical_relationship","label":"These renal junction proteins work together.","description":"Claudin-16/19 coexpression increases paracellular cation selectivity in LLC-PK1 epithelial monolayers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b9e564ce-7834-5815-a22b-0aa555a7bfe4","slug":"cldn16","display_name":"Claudin-16","entity_type_key":"protein"},"role":"junction_component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2e41ca25-b273-5974-8e13-cfa2654da636","slug":"cldn19","display_name":"Claudin-19","entity_type_key":"protein"},"role":"junction_component","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Claudin expression in LLC-PK1/MDCK epithelia; interaction and permeability assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Effects differ in MDCK cells; cation selectivity does not prove a calcium-specific pore.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Heterologous renal epithelial systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"These renal junction proteins work together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hou2008] Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2176193/ DOI: 10.1172/JCI33970","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal tight-junction model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0df6fa7f-5129-557e-965b-371e5de4cef5","evidence_kind":"source_excerpt","locator":"Lines 258-267","start_line":258,"end_line":267,"excerpt":"### cldn16-cldn19-cation-selectivity\nClaudin-16/19 coexpression increases paracellular cation selectivity in LLC-PK1 epithelial monolayers.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: These renal junction proteins work together.\norganism: Heterologous renal epithelial systems\ntissue_or_cell_type: Renal tight-junction model\nexperimental_model: Claudin expression in LLC-PK1/MDCK epithelia; interaction and permeability assays\nlimitations: Effects differ in MDCK cells; cation selectivity does not prove a calcium-specific pore.\n[hou2008] Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2176193/ DOI: 10.1172/JCI33970","model_system":"Claudin expression in LLC-PK1/MDCK epithelia; interaction and permeability assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hou2008] Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2176193/ DOI: 10.1172/JCI33970","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}