{"id":"c102d7b5-a6e3-55d8-84bf-4bf62a4d2732","stable_key":"8ccb5461-efe2-5d95-9869-950d7cd50575:cyclic-amp-does-not-substitute-for-the-cgmp-route","predicate":"no_detected_effect_on","statement":"Raising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"3b810eb0-aa66-5c35-90ce-7d8c2f880b40","mechanism_event_label":"Raising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.","subject":{"id":"4c288678-9116-5460-a9a9-8f7e1f253430","slug":"prkg1","display_name":"cGMP-dependent protein kinase I","entity_type_key":"protein"},"object":{"id":"a0f443db-03a6-5fc6-bdaf-f83d943458de","slug":"corpus-cavernosum-relaxation","display_name":"Corpus cavernosum smooth muscle relaxation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3b810eb0-aa66-5c35-90ce-7d8c2f880b40","stable_key":"8ccb5461-efe2-5d95-9869-950d7cd50575:cyclic-amp-does-not-substitute-for-the-cgmp-route-event","event_type":"observed_relationship","label":"Raising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.","description":"Raising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4c288678-9116-5460-a9a9-8f7e1f253430","slug":"prkg1","display_name":"cGMP-dependent protein kinase I","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a0f443db-03a6-5fc6-bdaf-f83d943458de","slug":"corpus-cavernosum-relaxation","display_name":"Corpus cavernosum smooth muscle relaxation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6d2f509b-3a5b-5a38-b3e7-b148581edec9","slug":"cgmp","display_name":"Cyclic guanosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"cGMP-dependent kinase I null mice compared with normal mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Forskolin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Shows an intact parallel cAMP route in isolated tissue; the authors conclude cAMP signalling cannot compensate for the absent cGMP cascade in vivo, which is an inference from the reproductive phenotype rather than a separate measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"cGMP-dependent kinase I null mice compared with normal mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Raising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Erectile dysfunction in cyclic GMP-dependent kinase I-deficient mice. (2000). https://pubmed.ncbi.nlm.nih.gov/10688876/ DOI: 10.1073/pnas.030419997","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Isolated tissue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Corpus cavernosum smooth muscle relaxation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fd564830-2fb3-503e-a9e3-30207c60d8f8","evidence_kind":"source_excerpt","locator":"Lines 67-76","start_line":67,"end_line":76,"excerpt":"## cyclic-amp-does-not-substitute-for-the-cgmp-route\nRaising cyclic AMP with forskolin produced similar relaxation in normal and cGMP-dependent kinase I null corpus cavernosum.\nModel/species: cGMP-dependent kinase I null mice compared with normal mice\nTissue/system: Corpus cavernosum smooth muscle relaxation\nExposure: Forskolin\nRoute: Isolated tissue\nDuration: Acute\nLimits: Shows an intact parallel cAMP route in isolated tissue; the authors conclude cAMP signalling cannot compensate for the absent cGMP cascade in vivo, which is an inference from the reproductive phenotype rather than a separate measurement.\nPrimary reference: Erectile dysfunction in cyclic GMP-dependent kinase I-deficient mice. (2000). https://pubmed.ncbi.nlm.nih.gov/10688876/ DOI: 10.1073/pnas.030419997\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"cca24ae7-e036-525c-a440-4cc2e38ccb4d","stable_key":"import-8ccb5461-efe2-5d95-9869-950d7cd50575","title":"Tadalafil: PDE5 occupancy, the cGMP route and three measured endpoints (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of seven primary studies resolved by PubMed title search, with every abstract read and all DOIs cross-checked against live PubMed metadata. Two references carry an unresolvable erratum and are recorded as corrected. Study-specific doses, effect sizes and limitations retained. Not publisher full text.","file_path":"","sha256":"3df8ec8f7114f983e8cae9fbc54912d3ac5b5f962454afea9dd8d4438c94cded","revision_id":"510acdea-65ae-57a6-b743-68e031ff1f05","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}