{"id":"c0df8b81-4f65-58cc-8cbd-4fab8a4bb995","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-bcrp-inhibition","predicate":"inhibits","statement":"Tartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2fd2dcbe-897a-50a9-ae17-36bc2193f073","mechanism_event_label":"Tartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"a52e10c2-fd56-564b-8c05-27e9fce27cd6","slug":"abcg2","display_name":"Human ABCG2 / breast cancer resistance protein","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2fd2dcbe-897a-50a9-ae17-36bc2193f073","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-bcrp-inhibition-event","event_type":"observed_relationship","label":"Tartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.","description":"Tartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a52e10c2-fd56-564b-8c05-27e9fce27cd6","slug":"abcg2","display_name":"Human ABCG2 / breast cancer resistance protein","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Food-additive screen at 50 micromolar; concentration-response experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Exact incubation interval not recovered from accessed primary material","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and publisher supplementary screening table; assay detail gaps retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ABCG2 / BCRP transporter in membrane-vesicle assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary abstract and publisher supplementary screening table inspected; no unverified IC50 assigned. Vesicle inhibition does not establish increased drug absorption, vitamin depletion or inhibition of every ABC transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human ABCG2 / BCRP transporter in membrane-vesicle assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interaction of Food Additives with Intestinal Efflux Transporters. (2017). https://pubmed.ncbi.nlm.nih.gov/28921988/ DOI: 10.1021/acs.molpharmaceut.7b00563","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro exposure to membrane vesicles","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Probe-substrate efflux","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"45353c4f-94dd-5c12-aa0a-7a1d3879971d","evidence_kind":"source_excerpt","locator":"Lines 215-224","start_line":215,"end_line":224,"excerpt":"## tartrazine-bcrp-inhibition\nTartrazine inhibited human BCRP-mediated probe transport in the membrane-vesicle screen.\nModel/species: Human ABCG2 / BCRP transporter in membrane-vesicle assay\nTissue: Probe-substrate efflux\nExposure: Food-additive screen at 50 micromolar; concentration-response experiments\nRoute: In vitro exposure to membrane vesicles\nDuration: Exact incubation interval not recovered from accessed primary material\nLimits: Primary abstract and publisher supplementary screening table inspected; no unverified IC50 assigned. Vesicle inhibition does not establish increased drug absorption, vitamin depletion or inhibition of every ABC transporter.\nPrimary reference: Interaction of Food Additives with Intestinal Efflux Transporters. (2017). https://pubmed.ncbi.nlm.nih.gov/28921988/ DOI: 10.1021/acs.molpharmaceut.7b00563\nAccess: Primary PubMed abstract and publisher supplementary screening table; assay detail gaps retained.","model_system":"Human ABCG2 / BCRP transporter in membrane-vesicle assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"b83a0113a4b743adc91c24504e488f47e10665386b299c4cb0543da8948f4246","revision_id":"3769070e-6af4-5b02-9d8e-f23c84edba1b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}