{"id":"c0a38399-f932-5657-bc15-0eafe5d2b63f","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-nka-potassium-dephosphorylation","predicate":"stimulates","statement":"Adding KCl after ATP phosphorylation accelerated dephosphorylation of pig-kidney Na/K-ATPase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"04e9b400-6db3-5044-9126-b224715f49cb","mechanism_event_label":"Potassium promotes the phosphate-removal part of the pump cycle.","subject":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"object":{"id":"37d1bc64-c915-59d7-b5d0-92ea1752c2ec","slug":"sodium-potassium-atpase-dephosphorylation","display_name":"Sodium-potassium ATPase dephosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"04e9b400-6db3-5044-9126-b224715f49cb","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-nka-potassium-dephosphorylation-event","event_type":"biochemical_relationship","label":"Potassium promotes the phosphate-removal part of the pump cycle.","description":"Adding KCl after ATP phosphorylation accelerated dephosphorylation of pig-kidney Na/K-ATPase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"earlier phosphorylation substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"catalytic-cycle cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"37d1bc64-c915-59d7-b5d0-92ea1752c2ec","slug":"sodium-potassium-atpase-dephosphorylation","display_name":"Sodium-potassium ATPase dephosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium-dependent ATP chemistry is coupled to sodium and potassium handling by the pump; serum magnesium is not the enzyme-site concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pig-kidney enzyme stopped-flow RH421 fluorescence; sequential ATP then KCl mixing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Stopped-flow assay; no dietary or magnesium-depletion intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Potassium promotes the phosphate-removal part of the pump cycle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kane-1998-nka] Dephosphorylation kinetics of pig kidney Na+,K+-ATPase (1998). https://pubmed.ncbi.nlm.nih.gov/9521778/ DOI: 10.1021/bi972813e","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb90d808-4eeb-506f-aa88-daf5332edbce","evidence_kind":"source_excerpt","locator":"Lines 807-817","start_line":807,"end_line":817,"excerpt":"### mg-nka-potassium-dephosphorylation\nAdding KCl after ATP phosphorylation accelerated dephosphorylation of pig-kidney Na/K-ATPase.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Potassium promotes the phosphate-removal part of the pump cycle.\norganism: Sus scrofa\ntissue_or_cell_type: Kidney enzyme\nexperimental_model: Pig-kidney enzyme stopped-flow RH421 fluorescence; sequential ATP then KCl mixing.\nlimitations: Stopped-flow assay; no dietary or magnesium-depletion intervention.\ncross_nutrient: Magnesium-dependent ATP chemistry is coupled to sodium and potassium handling by the pump; serum magnesium is not the enzyme-site concentration.\n[kane-1998-nka] Dephosphorylation kinetics of pig kidney Na+,K+-ATPase (1998). https://pubmed.ncbi.nlm.nih.gov/9521778/ DOI: 10.1021/bi972813e","model_system":"Pig-kidney enzyme stopped-flow RH421 fluorescence; sequential ATP then KCl mixing.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [kane-1998-nka] Dephosphorylation kinetics of pig kidney Na+,K+-ATPase (1998). https://pubmed.ncbi.nlm.nih.gov/9521778/ DOI: 10.1021/bi972813e","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}