{"id":"c08d91a8-40f8-5dca-a49e-a2cdcb1cf71b","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-l6-transport","predicate":"stimulates","statement":"Vanadate and pervanadate stimulated glucose transport and GLUT movement to the plasma membrane in rat L6 myotubes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"721cc396-aa05-552e-acee-c6a842369cd5","mechanism_event_label":"More transporters at the surface can increase glucose entry.","subject":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"object":{"id":"6efe3403-195d-5d5c-b94e-5df545d4a772","slug":"rat-l6-vanadate-glucose-transport","display_name":"Glucose transport in vanadate-treated rat L6 myotubes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"721cc396-aa05-552e-acee-c6a842369cd5","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-l6-transport-event","event_type":"observed_relationship","label":"More transporters at the surface can increase glucose entry.","description":"Vanadate and pervanadate stimulated glucose transport and GLUT movement to the plasma membrane in rat L6 myotubes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6efe3403-195d-5d5c-b94e-5df545d4a772","slug":"rat-l6-vanadate-glucose-transport","display_name":"Glucose transport in vanadate-treated rat L6 myotubes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f1faad21-091c-5b03-a03c-a20011d36fef","slug":"pervanadate","display_name":"Pervanadate / peroxovanadium(V) assay species","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b355feba-1bd3-55d6-9f12-54b9fbadfd58","slug":"rat-slc2a4","display_name":"Rat glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat L6 myotubes; related rat H9c2 experiments used tagged GLUT4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"GLUT isoforms in the L6 result are not all resolved in the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"More transporters at the surface can increase glucose entry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Tyrosine phosphatase inhibitors, vanadate and pervanadate, stimulate glucose transport and GLUT translocation in muscle cells by a mechanism independent of phosphatidylinositol 3-kinase and protein kinase C. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9792535/ · DOI 10.2337/diabetes.47.11.1676","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be9ccaf8-82c1-5ed3-b8f8-e18d202a5b20","evidence_kind":"source_excerpt","locator":"Lines 54-60","start_line":54,"end_line":60,"excerpt":"## vanadium-l6-transport\nMore transporters at the surface can increase glucose entry.\nVanadate and pervanadate stimulated glucose transport and GLUT movement to the plasma membrane in rat L6 myotubes.\nModel: Rat L6 myotubes; related rat H9c2 experiments used tagged GLUT4.\nLimitations: GLUT isoforms in the L6 result are not all resolved in the accessed abstract.\nEvidence access: Primary abstract\nTyrosine phosphatase inhibitors, vanadate and pervanadate, stimulate glucose transport and GLUT translocation in muscle cells by a mechanism independent of phosphatidylinositol 3-kinase and protein kinase C. · 1998 · https://pubmed.ncbi.nlm.nih.gov/9792535/ · DOI 10.2337/diabetes.47.11.1676","model_system":"Rat L6 myotubes; related rat H9c2 experiments used tagged GLUT4.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"d36b30d972e756ca4d19b66f976980e206c65025bd4bbf4c127c17320bd019c2","revision_id":"70d42967-8032-5627-8670-f59841735e89","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}