{"id":"c07bb36e-92fc-55d4-9886-4d90686917a7","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-vitamin-c-demethylation","predicate":"maternal_depletion_reduces","statement":"Maternal vitamin C withdrawal increased Dazl-promoter methylation in female fetal germ cells, with reduced Dazl expression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"55c5210a-1f4f-5834-a549-700f77bd7451","mechanism_event_label":"Vitamin C shortage altered an epigenetic step in germ-cell development.","subject":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"object":{"id":"b5563204-fe54-540d-8fc7-74474b94880e","slug":"mouse-dazl-promoter-demethylation","display_name":"Mouse Dazl promoter demethylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"55c5210a-1f4f-5834-a549-700f77bd7451","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-vitamin-c-demethylation-event","event_type":"biochemical_relationship","label":"Vitamin C shortage altered an epigenetic step in germ-cell development.","description":"Maternal vitamin C withdrawal increased Dazl-promoter methylation in female fetal germ cells, with reduced Dazl expression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a78afdc4-0484-522c-8047-c9df778c9aeb","slug":"mouse-dazl-gene","display_name":"Mouse Dazl gene","entity_type_key":"gene"},"role":"measured_locus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"be39243b-1d20-58c9-9f76-1480560334ac","slug":"tet1-mouse","display_name":"Mouse Tet1","entity_type_key":"protein"},"role":"related_demethylation_machinery","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2047867f-9872-53b2-a553-71c911d0695b","slug":"ascorbic-acid","display_name":"L-Ascorbic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b5563204-fe54-540d-8fc7-74474b94880e","slug":"mouse-dazl-promoter-demethylation","display_name":"Mouse Dazl promoter demethylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Same-genotype nutritional comparison and bisulfite sequencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Gulo-null dams: water vitamin C withdrawn 3-7 days before mating through E13.5; controls 3.3 g/L.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Methylation-expression association does not prove the sole causal mediator or impaired RA synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin C shortage altered an epigenetic step in germ-cell development.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ditroia2019] Maternal vitamin C regulates reprogramming of DNA methylation and germline development. (2019). https://pubmed.ncbi.nlm.nih.gov/31485074/ DOI: 10.1038/s41586-019-1536-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"female fetal germ cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"83102917-410b-5fd7-afa7-3915739a418a","evidence_kind":"source_excerpt","locator":"Lines 369-380","start_line":369,"end_line":380,"excerpt":"### va-repro-vitamin-c-demethylation\nMaternal vitamin C withdrawal increased Dazl-promoter methylation in female fetal germ cells, with reduced Dazl expression.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin C shortage altered an epigenetic step in germ-cell development.\norganism: Mus musculus\ntissue_or_cell_type: female fetal germ cells\nexperimental_model: Same-genotype nutritional comparison and bisulfite sequencing\nlimitations: Methylation-expression association does not prove the sole causal mediator or impaired RA synthesis.\nexposure: Gulo-null dams: water vitamin C withdrawn 3-7 days before mating through E13.5; controls 3.3 g/L.\ncross_nutrient: true\n[ditroia2019] Maternal vitamin C regulates reprogramming of DNA methylation and germline development. (2019). https://pubmed.ncbi.nlm.nih.gov/31485074/ DOI: 10.1038/s41586-019-1536-1","model_system":"Same-genotype nutritional comparison and bisulfite sequencing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ditroia2019] Maternal vitamin C regulates reprogramming of DNA methylation and germline development. (2019). https://pubmed.ncbi.nlm.nih.gov/31485074/ DOI: 10.1038/s41586-019-1536-1","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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