{"id":"c0026ceb-8665-5c06-8fac-2b74964bbe35","stable_key":"8c094dea-83bb-53f1-8ad6-2780b4f131eb:minus-lariciresinol-regulates-hnf1-alpha","predicate":"regulates","statement":"Combining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8ef923be-2f3e-5a35-bdf8-17313aec20b1","mechanism_event_label":"Combining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.","subject":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"object":{"id":"c9b0f8e4-4823-5067-a352-61b4c37cd11b","slug":"hnf1a","display_name":"Human hepatocyte nuclear factor 1 alpha / HNF1A","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8ef923be-2f3e-5a35-bdf8-17313aec20b1","stable_key":"8c094dea-83bb-53f1-8ad6-2780b4f131eb:minus-lariciresinol-regulates-hnf1-alpha-event","event_type":"observed_relationship","label":"Combining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.","description":"Combining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c9b0f8e4-4823-5067-a352-61b4c37cd11b","slug":"hnf1a","display_name":"Human hepatocyte nuclear factor 1 alpha / HNF1A","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b6b0e047-df6c-5f77-a751-51019c928484","slug":"hbv-rna-production","display_name":"Hepatitis B virus RNA production","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hepatoma cells carrying hepatitis B virus, with RNA sequencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"(-)-Lariciresinol isolated from the roots of Isatis indigotica","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract hedges the link as one that may be related, and states regulation without giving a direction, a magnitude or a concentration, so no direction is recorded. No knockdown or over-expression rescue of HNF1α is described in the abstract, so the relationship is associative rather than demonstrated as necessary.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Hepatoma cells carrying hepatitis B virus, with RNA sequencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Combining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"(-)-Lariciresinol Isolated from the Roots of Isatis indigotica Fortune ex Lindl. Inhibits Hepatitis B Virus by Regulating Viral Transcription. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103223","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Host transcription factor and viral transcriptional activity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c33494a-bc56-5154-a44b-86c3441631ab","evidence_kind":"source_excerpt","locator":"Lines 11-20","start_line":11,"end_line":20,"excerpt":"## minus-lariciresinol-regulates-hnf1-alpha\nCombining RNA sequencing analysis, the decrease in hepatitis B virus transcriptional activity by (-)-lariciresinol was reported as possibly related to hepatocyte nuclear factor 1 alpha, and the compound was described as inhibiting replication by regulating HNF1α-mediated transcription.\nModel/species: Hepatoma cells carrying hepatitis B virus, with RNA sequencing\nTissue/system: Host transcription factor and viral transcriptional activity\nExposure: (-)-Lariciresinol isolated from the roots of Isatis indigotica\nRoute: In vitro\nDuration: Not stated here\nLimits: The abstract hedges the link as one that may be related, and states regulation without giving a direction, a magnitude or a concentration, so no direction is recorded. No knockdown or over-expression rescue of HNF1α is described in the abstract, so the relationship is associative rather than demonstrated as necessary.\nPrimary reference: (-)-Lariciresinol Isolated from the Roots of Isatis indigotica Fortune ex Lindl. Inhibits Hepatitis B Virus by Regulating Viral Transcription. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103223\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9ca4f4ed-6090-5e7d-9327-81eae8924fcb","stable_key":"import-8c094dea-83bb-53f1-8ad6-2780b4f131eb","title":"(-)-Lariciresinol and hepatocyte nuclear factor 1 alpha: the host-factor step (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"f226f699d178d9ae63fdf6e5d80ae0077b7e5bfda4e0683b836fc057a7ac1eca","revision_id":"4503344a-a294-5dd8-bff9-c2966f2464cb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}