{"id":"bff44500-10bb-5704-8c51-09bb69e5f4c8","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-endogenous-iron-loss","predicate":"restrains-deficiency-associated","statement":"After intraperitoneal 59Fe, riboflavin-deficient rats lost about twice as much endogenous tracer in feces as weight-matched controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c0d463fd-e7af-5eb8-a308-5b0f096bd539","mechanism_event_label":"Deficiency increased loss of iron already inside the body.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"a3d30453-f0df-520a-bf1a-0267aee0770e","slug":"endogenous-fecal-iron-loss","display_name":"Endogenous fecal iron loss","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c0d463fd-e7af-5eb8-a308-5b0f096bd539","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-rat-endogenous-iron-loss-event","event_type":"biochemical_relationship","label":"Deficiency increased loss of iron already inside the body.","description":"After intraperitoneal 59Fe, riboflavin-deficient rats lost about twice as much endogenous tracer in feces as weight-matched controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"endogenous radiotracer","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a3d30453-f0df-520a-bf1a-0267aee0770e","slug":"endogenous-fecal-iron-loss","display_name":"Endogenous fecal iron loss","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"B2 status affected post-absorption iron retention in rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Riboflavin-deficient weanling rats; weight-matched and ad-libitum controls; intestinal histology and intraperitoneal 59Fe tracing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary riboflavin deficiency plus intraperitoneal tracer","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The proposed enterocyte-turnover mechanism is supported indirectly; this is not measurement of unabsorbed oral iron.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Deficiency increased loss of iron already inside the body.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[powers1993] A proposed intestinal mechanism for the effect of riboflavin deficiency on iron loss in the rat. (1993). https://pubmed.ncbi.nlm.nih.gov/8490008/ DOI: 10.1079/bjn19930055","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Gastrointestinal tract and feces","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fc5c4d5b-c2bc-5bd9-b73b-462f6f8e42c4","evidence_kind":"source_excerpt","locator":"Lines 1458-1470","start_line":1458,"end_line":1470,"excerpt":"### b2-rat-endogenous-iron-loss\nAfter intraperitoneal 59Fe, riboflavin-deficient rats lost about twice as much endogenous tracer in feces as weight-matched controls.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Deficiency increased loss of iron already inside the body.\norganism: Rattus norvegicus\ntissue_or_cell_type: Gastrointestinal tract and feces\nexperimental_model: Riboflavin-deficient weanling rats; weight-matched and ad-libitum controls; intestinal histology and intraperitoneal 59Fe tracing.\nlimitations: The proposed enterocyte-turnover mechanism is supported indirectly; this is not measurement of unabsorbed oral iron.\nexposure: Dietary riboflavin deficiency plus intraperitoneal tracer\ncross_nutrient: B2 status affected post-absorption iron retention in rats.\nevidence_location: Abstract\n[powers1993] A proposed intestinal mechanism for the effect of riboflavin deficiency on iron loss in the rat. (1993). https://pubmed.ncbi.nlm.nih.gov/8490008/ DOI: 10.1079/bjn19930055","model_system":"Riboflavin-deficient weanling rats; weight-matched and ad-libitum controls; intestinal histology and intraperitoneal 59Fe tracing.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [powers1993] A proposed intestinal mechanism for the effect of riboflavin deficiency on iron loss in the rat. (1993). https://pubmed.ncbi.nlm.nih.gov/8490008/ DOI: 10.1079/bjn19930055","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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