{"id":"bf9c248d-0689-57fd-aabe-692fabc50cea","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-sod2-ab-null","predicate":"does_not_significantly_change","statement":"ERG a- and b-wave amplitudes did not differ between supplemented and control knockout mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d477c497-3364-52d5-9320-a22ece187eec","mechanism_event_label":"Protection did not extend to every retinal function measure.","subject":{"id":"0a60c685-7502-5ece-9da2-2c1a2e3bcdf1","slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"},"object":{"id":"095dddcd-30fa-5838-b0bb-e282c9dfa798","slug":"mouse-retinal-a-b-waves","display_name":"Mouse ERG a- and b-wave amplitudes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d477c497-3364-52d5-9320-a22ece187eec","stable_key":"d8050016-13c9-5391-9d61-63de73590053:zeaxanthin-sod2-ab-null-event","event_type":"biochemical_relationship","label":"Protection did not extend to every retinal function measure.","description":"ERG a- and b-wave amplitudes did not differ between supplemented and control knockout mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0a60c685-7502-5ece-9da2-2c1a2e3bcdf1","slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"095dddcd-30fa-5838-b0bb-e282c9dfa798","slug":"mouse-retinal-a-b-waves","display_name":"Mouse ERG a- and b-wave amplitudes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zeaxanthin-research/30265681.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"656f827109394a907278c44e8d33e883c92437beea2a607e99e75ae348489151\", \"start_char\": 0, \"end_char\": 2098, \"text_sha256\": \"656f827109394a907278c44e8d33e883c92437beea2a607e99e75ae348489151\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"RPE-specific knockout with oral supplementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"55–60 mg/kg/day zeaxanthin by gavage for four months","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High-dose preventive animal model; genetic Sod2 loss is not manganese dietary deficiency, and this does not establish human AMD treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zeaxanthin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zeaxanthin","display_name":"Dietary (3R,3-prime-R)-zeaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Sod2-deleted mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Protection did not extend to every retinal function measure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zeaxanthin-p30265681] Daily zeaxanthin supplementation prevents atrophy of the retinal pigment epithelium (RPE) in a mouse model of mitochondrial oxidative stress. 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