{"id":"bf6aff89-2057-53a7-bfb0-3c1a2260cda1","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-hcg-gate","predicate":"potentiates_tested","statement":"In mouse MA-10 cells, D-aspartate alone did not meaningfully increase measured testosterone; 0.1 or 1 nM D-aspartate augmented the hCG-stimulated immunoassay signal.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bc10fd7b-3d09-5e30-becc-52728bde2347","mechanism_event_label":"The response required the hormone context in this cell line.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"d8c512b6-c146-5c4b-ab73-5672c768d860","slug":"mouse-ma10-testosterone-immunoassay","display_name":"MA-10 medium testosterone immunoassay endpoint","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bc10fd7b-3d09-5e30-becc-52728bde2347","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-hcg-gate-event","event_type":"observed_relationship","label":"The response required the hormone context in this cell line.","description":"In mouse MA-10 cells, D-aspartate alone did not meaningfully increase measured testosterone; 0.1 or 1 nM D-aspartate augmented the hCG-stimulated immunoassay signal.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d8c512b6-c146-5c4b-ab73-5672c768d860","slug":"mouse-ma10-testosterone-immunoassay","display_name":"MA-10 medium testosterone immunoassay endpoint","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ee453fb-ad7f-54ca-ab03-6397b781901e","slug":"human-chorionic-gonadotropin","display_name":"Human chorionic gonadotropin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e3013e06-20da-5c91-b8f8-d9364a645fae","slug":"testosterone","display_name":"Testosterone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse Leydig tumor cell line; medium electrochemiluminescence immunoassay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line and assay-specific endpoint, not human testosterone efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The response required the hormone context in this cell line.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5e6a1ff3-bd16-5853-98b2-80abd0eea01d","evidence_kind":"source_excerpt","locator":"Lines 384-390","start_line":384,"end_line":390,"excerpt":"## d-aspartate-leydig-hcg-gate\nThe response required the hormone context in this cell line.\nIn mouse MA-10 cells, D-aspartate alone did not meaningfully increase measured testosterone; 0.1 or 1 nM D-aspartate augmented the hCG-stimulated immunoassay signal.\nModel: Mouse Leydig tumor cell line; medium electrochemiluminescence immunoassay.\nLimitations: Cell-line and assay-specific endpoint, not human testosterone efficacy.\nEvidence access: Primary abstract\nD-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","model_system":"Mouse Leydig tumor cell line; medium electrochemiluminescence immunoassay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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