{"id":"bf3f1dd3-f2a8-5055-bb29-5ebe1775036a","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc-a165t-location","predicate":"alters","statement":"Endogenous A165T MTARC1 showed substantial mislocalization outside the outer mitochondrial membrane in HepG2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"38a48f18-ade4-52f6-af24-f5e85cf9ceb0","mechanism_event_label":"The altered protein was not positioned like the usual enzyme.","subject":{"id":"7dc83e29-73ed-5de6-ba11-113190e5efa1","slug":"mtarc1-a165t","display_name":"Human MTARC1 p.Ala165Thr","entity_type_key":"protein_state"},"object":{"id":"e8a5ac21-d525-59ef-8b2e-19b1f656ead1","slug":"mtarc1-mitochondrial-localization","display_name":"Human MTARC1 outer mitochondrial membrane localization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"38a48f18-ade4-52f6-af24-f5e85cf9ceb0","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc-a165t-location-event","event_type":"biochemical_relationship","label":"The altered protein was not positioned like the usual enzyme.","description":"Endogenous A165T MTARC1 showed substantial mislocalization outside the outer mitochondrial membrane in HepG2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7dc83e29-73ed-5de6-ba11-113190e5efa1","slug":"mtarc1-a165t","display_name":"Human MTARC1 p.Ala165Thr","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e8a5ac21-d525-59ef-8b2e-19b1f656ead1","slug":"mtarc1-mitochondrial-localization","display_name":"Human MTARC1 outer mitochondrial membrane localization","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/38723751.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5c4b7e298ad8a9760c515d21299d8c831692c6c07361857e3f6ff6509e9438db\", \"start_char\": 0, \"end_char\": 1564, \"text_sha256\": \"5c4b7e298ad8a9760c515d21299d8c831692c6c07361857e3f6ff6509e9438db\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Endogenous CRISPR knock-in variants in HepG2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"A165T versus WT and catalytically inactive C273A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cellular protein effects; the protective mechanism in human liver disease was not proven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The altered protein was not positioned like the usual enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p38723751] Biochemical and functional characterization of the p.A165T missense variant of mitochondrial amidoxime-reducing component 1. (2024). https://pubmed.ncbi.nlm.nih.gov/38723751/ DOI: 10.1016/j.jbc.2024.107353","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"125844ed-cab0-5478-8d7e-28863793d74a","evidence_kind":"source_excerpt","locator":"Lines 1483-1494","start_line":1483,"end_line":1494,"excerpt":"### mo-marc-a165t-location\nEndogenous A165T MTARC1 showed substantial mislocalization outside the outer mitochondrial membrane in HepG2 cells.\nCondition category: machinery_impairment\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The altered protein was not positioned like the usual enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Hepatoma cells\nexperimental_model: Endogenous CRISPR knock-in variants in HepG2 cells\nlimitations: Cellular protein effects; the protective mechanism in human liver disease was not proven.\nexposure: A165T versus WT and catalytically inactive C273A\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/38723751.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5c4b7e298ad8a9760c515d21299d8c831692c6c07361857e3f6ff6509e9438db\", \"start_char\": 0, \"end_char\": 1564, \"text_sha256\": \"5c4b7e298ad8a9760c515d21299d8c831692c6c07361857e3f6ff6509e9438db\"}\n[mo-p38723751] Biochemical and functional characterization of the p.A165T missense variant of mitochondrial amidoxime-reducing component 1. 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