{"id":"bf365b87-65a3-5968-823f-1bfe178924ee","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-autophagy-becn1","predicate":"increases_expression","statement":"Indicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"09717333-882b-5593-8569-a4e935e03319","mechanism_event_label":"Indicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.","subject":{"id":"f2a6e469-05f0-5596-9054-da926389f584","slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"},"object":{"id":"6a93081b-9e7a-5029-80f6-c82c73d167dc","slug":"human-becn1","display_name":"Human Beclin 1 / BECN1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"09717333-882b-5593-8569-a4e935e03319","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-autophagy-becn1-event","event_type":"observed_relationship","label":"Indicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.","description":"Indicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f2a6e469-05f0-5596-9054-da926389f584","slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6a93081b-9e7a-5029-80f6-c82c73d167dc","slug":"human-becn1","display_name":"Human Beclin 1 / BECN1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Indicaxanthin 10, 50 and 100 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"48 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, relevant results/methods and PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 colorectal cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Marker abundance and acidic organelles are not sufficient alone to quantify complete autophagic flux. Predicted BCL2 binding remains computational; methylation associations do not prove causation. The protein response was not monotonic across the tested concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Dedicated indicaxanthin chapter; original betalain family identity and shared claims preserved.","comparator":null,"unit":null,"notes":"","entity":{"slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human Caco-2 colorectal cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Indicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Indicaxanthin Induces Autophagy in Intestinal Epithelial Cancer Cells by Epigenetic Mechanisms Involving DNA Methylation. (2023). https://pubmed.ncbi.nlm.nih.gov/37571432/ DOI: 10.3390/nu15153495","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Autophagy markers and DNA methylome","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8c976792-ad39-5b92-a221-ef3735e9968e","evidence_kind":"source_excerpt","locator":"Lines 426-435","start_line":426,"end_line":435,"excerpt":"## indicaxanthin-autophagy-becn1\nIndicaxanthin at 50 micromolar increased Beclin1 protein abundance in Caco-2 cells.\nModel/species: Human Caco-2 colorectal cancer cells\nTissue: Autophagy markers and DNA methylome\nExposure: Indicaxanthin 10, 50 and 100 micromolar\nRoute: In vitro addition\nDuration: 48 h\nLimits: Marker abundance and acidic organelles are not sufficient alone to quantify complete autophagic flux. Predicted BCL2 binding remains computational; methylation associations do not prove causation. The protein response was not monotonic across the tested concentrations.\nPrimary reference: Indicaxanthin Induces Autophagy in Intestinal Epithelial Cancer Cells by Epigenetic Mechanisms Involving DNA Methylation. (2023). https://pubmed.ncbi.nlm.nih.gov/37571432/ DOI: 10.3390/nu15153495\nAccess: Primary open full text, relevant results/methods and PubMed metadata.","model_system":"Human Caco-2 colorectal cancer cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived curation span; original paraphrase with primary citation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"18b7303c-98b7-54cb-ae8e-a8c6257657b1","stable_key":"import-c94a9824-35fb-523c-b421-dc7341218dcb","title":"Indicaxanthin: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen additional primary studies, with twenty-six existing claims from eight studies linked unchanged. Study-specific citations and limitations retained. 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