{"id":"bf3581ef-ff7f-5794-8d7c-8ef36c2800ef","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-a39-independent","predicate":"supports","statement":"An independent CRISPR/transport study identified SLC25A39 as critical for mitochondrial GSH import.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"837ee222-e0a2-5056-9f94-44097fab9377","mechanism_event_label":"A second study supported the carrier assignment.","subject":{"id":"2b210ef5-c77e-57a3-be04-7876c67b8d75","slug":"slc25a39","display_name":"Human mitochondrial glutathione carrier SLC25A39","entity_type_key":"protein"},"object":{"id":"8b403d22-564a-5694-bdfc-31377566b2e5","slug":"mitochondrial-gsh-import","display_name":"Mammalian mitochondrial glutathione import","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"837ee222-e0a2-5056-9f94-44097fab9377","stable_key":"db0fc92e-b5ef-5667-a4c5-3ef257edbc9b:glutathione-a39-independent-event","event_type":"biochemical_relationship","label":"A second study supported the carrier assignment.","description":"An independent CRISPR/transport study identified SLC25A39 as critical for mitochondrial GSH import.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"transported_metabolite","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b210ef5-c77e-57a3-be04-7876c67b8d75","slug":"slc25a39","display_name":"Human mitochondrial glutathione carrier SLC25A39","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8b403d22-564a-5694-bdfc-31377566b2e5","slug":"mitochondrial-gsh-import","display_name":"Mammalian mitochondrial glutathione import","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/glutathione-research/35513392.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"000d49d8bad7e173d95c214f6cb2bf2ea84b9e4af231687687d1aa703cdeaff7\", \"start_char\": 0, \"end_char\": 1457, \"text_sha256\": \"000d49d8bad7e173d95c214f6cb2bf2ea84b9e4af231687687d1aa703cdeaff7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Paired CRISPR screen and mitochondrial transport assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"2016 single/pair perturbations across four metabolic states","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic buffering depends on metabolic conditions; it is not evidence that iron supplementation corrects low GSH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glutathione research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human experimental cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A second study supported the carrier assignment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[glutathione-p35513392] Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS. (2022). https://pubmed.ncbi.nlm.nih.gov/35513392/ DOI: 10.1038/s41467-022-30126-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mitochondrial transport and respiration","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"40e00c2e-4cdd-5724-9ce5-cae4ae87d4e5","evidence_kind":"source_excerpt","locator":"Lines 515-526","start_line":515,"end_line":526,"excerpt":"### glutathione-a39-independent\nAn independent CRISPR/transport study identified SLC25A39 as critical for mitochondrial GSH import.\nCondition category: normal\nnutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A second study supported the carrier assignment.\norganism: Human experimental cells\ntissue_or_cell_type: Mitochondrial transport and respiration\nexperimental_model: Paired CRISPR screen and mitochondrial transport assays\nlimitations: Genetic buffering depends on metabolic conditions; it is not evidence that iron supplementation corrects low GSH.\nexposure: 2016 single/pair perturbations across four metabolic states\nevidence_span: {\"source_cache\": \"artifacts/glutathione-research/35513392.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"000d49d8bad7e173d95c214f6cb2bf2ea84b9e4af231687687d1aa703cdeaff7\", \"start_char\": 0, \"end_char\": 1457, \"text_sha256\": \"000d49d8bad7e173d95c214f6cb2bf2ea84b9e4af231687687d1aa703cdeaff7\"}\n[glutathione-p35513392] Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS. (2022). https://pubmed.ncbi.nlm.nih.gov/35513392/ DOI: 10.1038/s41467-022-30126-9","model_system":"Paired CRISPR screen and mitochondrial transport assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [glutathione-p35513392] Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS. (2022). https://pubmed.ncbi.nlm.nih.gov/35513392/ DOI: 10.1038/s41467-022-30126-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9b7417fd-059d-5683-b452-71d1e3cf82d0","stable_key":"import-db0fc92e-b5ef-5667-a4c5-3ef257edbc9b","title":"Glutathione: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2a5740c4a7e770aafe883fea47909b18bf5e174f211749935130a50f9dea2753","revision_id":"a64016dd-4b72-5954-a72d-0e61de854073","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}