{"id":"bf2e67cf-79b6-5848-8bdc-a9e57bf7dd0a","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:glucoside-lowers-influenza-induced-nf-kb-activation","predicate":"reduces","statement":"Lariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4bf670e3-73a6-5ea1-b7ee-069a67f817a5","mechanism_event_label":"Lariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.","subject":{"id":"6f5b3b44-5ea8-5441-8653-89a548721566","slug":"lariciresinol-4-o-glucoside","display_name":"Lariciresinol-4-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"object":{"id":"68f7561d-1a53-540c-8380-d544e5f4938d","slug":"influenza-nf-kb-reporter-activity","display_name":"NF-kappa-B reporter activity under influenza or cytokine stimulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4bf670e3-73a6-5ea1-b7ee-069a67f817a5","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:glucoside-lowers-influenza-induced-nf-kb-activation-event","event_type":"observed_relationship","label":"Lariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.","description":"Lariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f5b3b44-5ea8-5441-8653-89a548721566","slug":"lariciresinol-4-o-glucoside","display_name":"Lariciresinol-4-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"68f7561d-1a53-540c-8380-d544e5f4938d","slug":"influenza-nf-kb-reporter-activity","display_name":"NF-kappa-B reporter activity under influenza or cytokine stimulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK-293 cells stably carrying an NF-κB responsive firefly luciferase reporter, and A549 cells infected with influenza A virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lariciresinol-4-O-β-D-glucopyranoside isolated from the root of Isatis indigotica","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A reporter readout rather than a direct measurement on any NF-κB subunit; no inhibitory concentration for the reporter is given.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"HEK-293 cells stably carrying an NF-κB responsive firefly luciferase reporter, and A549 cells infected with influenza A virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lariciresinol-4-O-β-D-glucopyranoside from the root of Isatis indigotica inhibits influenza A virus-induced pro-inflammatory response. (2015). https://pubmed.ncbi.nlm.nih.gov/26320688/ DOI: 10.1016/j.jep.2015.08.037","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"NF-κB reporter activity and cytokine output","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"de650cbc-19fa-5b79-a906-7b9fdfe45236","evidence_kind":"source_excerpt","locator":"Lines 110-119","start_line":110,"end_line":119,"excerpt":"## glucoside-lowers-influenza-induced-nf-kb-activation\nLariciresinol-4-O-β-D-glucopyranoside attenuated NF-κB activation in a reporter cell line and reduced influenza-induced cytokine production in lung epithelial cells.\nModel/species: HEK-293 cells stably carrying an NF-κB responsive firefly luciferase reporter, and A549 cells infected with influenza A virus\nTissue/system: NF-κB reporter activity and cytokine output\nExposure: Lariciresinol-4-O-β-D-glucopyranoside isolated from the root of Isatis indigotica\nRoute: In vitro\nDuration: Not stated here\nLimits: A reporter readout rather than a direct measurement on any NF-κB subunit; no inhibitory concentration for the reporter is given.\nPrimary reference: Lariciresinol-4-O-β-D-glucopyranoside from the root of Isatis indigotica inhibits influenza A virus-induced pro-inflammatory response. (2015). https://pubmed.ncbi.nlm.nih.gov/26320688/ DOI: 10.1016/j.jep.2015.08.037\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}