{"id":"bf214b71-21c1-5300-b663-efab53252396","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-rat-pl-catabolism","predicate":"produces","statement":"Partially purified rat tissue NAD-dependent aldehyde-dehydrogenase activity oxidized PL to 4-pyridoxic acid; oxidase-null rats still excreted the acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"537b2bea-e269-517e-82bd-284625641bf7","mechanism_event_label":"Rat B6 breakdown had an alternative to aldehyde oxidase.","subject":{"id":"34a047f5-463c-5aba-89f6-c4b5d23a8fca","slug":"rat-nad-dependent-pyridoxal-dehydrogenase-activity","display_name":"Rat NAD-dependent pyridoxal dehydrogenase activity","entity_type_key":"cellular_process"},"object":{"id":"befbcf98-c6a9-5b72-a039-2d6b9f3bde12","slug":"4-pyridoxic-acid","display_name":"4-Pyridoxic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"537b2bea-e269-517e-82bd-284625641bf7","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-transport-rat-pl-catabolism-event","event_type":"biochemical_relationship","label":"Rat B6 breakdown had an alternative to aldehyde oxidase.","description":"Partially purified rat tissue NAD-dependent aldehyde-dehydrogenase activity oxidized PL to 4-pyridoxic acid; oxidase-null rats still excreted the acid.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9bf6269d-9e01-553d-bd8b-11a0d613bfca","slug":"pyridoxal","display_name":"Pyridoxal","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"electron acceptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"34a047f5-463c-5aba-89f6-c4b5d23a8fca","slug":"rat-nad-dependent-pyridoxal-dehydrogenase-activity","display_name":"Rat NAD-dependent pyridoxal dehydrogenase activity","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"befbcf98-c6a9-5b72-a039-2d6b9f3bde12","slug":"4-pyridoxic-acid","display_name":"4-Pyridoxic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"NAD links niacin-derived redox chemistry to B6 catabolism in this rat assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed abstract: partially purified rat enzyme and urinary metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat tissue enzyme fractions and aldehyde-oxidase activity variants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Partially purified activity; kinetic assays at pH 9.6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Molecular isoenzyme and quantitative contribution in humans not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Rat B6 breakdown had an alternative to aldehyde oxidase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[stanulovic1976] New pathway of conversion of pyridoxal to 4-pyridoxic acid. (1976). https://pubmed.ncbi.nlm.nih.gov/939227/ DOI: 10.1159/000458879","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rat tissue enzyme fractions and urine","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c4cc708e-f988-5a5a-ba9e-2e61627ed510","evidence_kind":"source_excerpt","locator":"Lines 491-503","start_line":491,"end_line":503,"excerpt":"### b6-transport-rat-pl-catabolism\nPartially purified rat tissue NAD-dependent aldehyde-dehydrogenase activity oxidized PL to 4-pyridoxic acid; oxidase-null rats still excreted the acid.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Rat B6 breakdown had an alternative to aldehyde oxidase.\norganism: Rattus norvegicus\ntissue_or_cell_type: Rat tissue enzyme fractions and urine\nexperimental_model: Rat tissue enzyme fractions and aldehyde-oxidase activity variants.\nlimitations: Molecular isoenzyme and quantitative contribution in humans not established.\nexposure: Partially purified activity; kinetic assays at pH 9.6.\nevidence_location: Indexed abstract: partially purified rat enzyme and urinary metabolites\ncross_nutrient: NAD links niacin-derived redox chemistry to B6 catabolism in this rat assay.\n[stanulovic1976] New pathway of conversion of pyridoxal to 4-pyridoxic acid. 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