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substrate incubations and HPLC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified BCO1 and alpha-carotene concentration series.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Lower catalytic efficiency than beta-carotene under these assay conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens protein expressed in Escherichia coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"outcome","value_text":"Human BCO1 generated retinal from alpha-carotene in substrate assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Alpha-carotene can feed retinal production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-delasena-2013] Substrate specificity of purified recombinant human beta-carotene 15,15'-oxygenase (BCO1) (2013). https://pubmed.ncbi.nlm.nih.gov/24187135/ DOI: 10.1074/jbc.M113.507160","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c0d820d2-b335-5dc2-849a-58ddf1535fe7","evidence_kind":"source_excerpt","locator":"Lines 145-157","start_line":145,"end_line":157,"excerpt":"### va-bco1-alpha-carotene\nHuman BCO1 generated retinal from alpha-carotene in substrate assays.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Alpha-carotene can feed retinal production.\norganism: Homo sapiens protein expressed in Escherichia coli\ntissue_or_cell_type: Cell-free enzyme\nexperimental_model: Purified human BCO1 expressed in E. coli; substrate incubations and HPLC.\nlimitations: Lower catalytic efficiency than beta-carotene under these assay conditions.\nexposure: Purified BCO1 and alpha-carotene concentration series.\noutcome: Human BCO1 generated retinal from alpha-carotene in substrate assays.\nevidence_location: Abstract\n[va-delasena-2013] Substrate specificity of purified recombinant human beta-carotene 15,15'-oxygenase (BCO1) (2013). https://pubmed.ncbi.nlm.nih.gov/24187135/ DOI: 10.1074/jbc.M113.507160","model_system":"Purified human BCO1 expressed in E. coli; substrate incubations and HPLC.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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