{"id":"be945d01-ae8c-5d42-8a77-f06a031dcaf0","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-sam-binding","predicate":"forms_complex_with","statement":"S-adenosylmethionine formed a boron complex in capillary-electrophoresis experiments and ranked among the strongest ligands tested.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"73b380b8-1851-50da-9206-4f8a85d0c467","mechanism_event_label":"Boron can attach to SAM in a test tube; this does not show that it supplies methyl groups or improves methylation.","subject":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"object":{"id":"8389b1b3-84ab-5f7b-981a-3bc125b1f25a","slug":"borate-sam-complex","display_name":"Boron complex with S-adenosylmethionine","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"73b380b8-1851-50da-9206-4f8a85d0c467","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-sam-binding-event","event_type":"biochemical_relationship","label":"Boron can attach to SAM in a test tube; this does not show that it supplies methyl groups or improves methylation.","description":"S-adenosylmethionine formed a boron complex in capillary-electrophoresis experiments and ranked among the strongest ligands tested.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"04e68eab-58b9-5be0-95b1-0739fc0699ff","slug":"borate","display_name":"Tetrahydroxyborate / B(OH)4−","entity_type_key":"ion"},"role":"binding_partner","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8389b1b3-84ab-5f7b-981a-3bc125b1f25a","slug":"borate-sam-complex","display_name":"Boron complex with S-adenosylmethionine","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/11420139.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0792ce09223d7cf23f0d3fda6b793e6e3b9904abe2c1f934b96d50e3a0e8b703\", \"start_char\": 0, \"end_char\": 1650, \"text_sha256\": \"0792ce09223d7cf23f0d3fda6b793e6e3b9904abe2c1f934b96d50e3a0e8b703\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Capillary electrophoresis of purified metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Boron complexation under the reported assay conditions","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chemical affinity is not a demonstrated metabolic function or in-vivo occupancy. Rankings depend on assay and solution conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Cell-free chemistry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Boron can attach to SAM in a test tube; this does not show that it supplies methyl groups or improves methylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p11420139] Diadenosine phosphates and S-adenosylmethionine: novel boron binding biomolecules detected by capillary electrophoresis. (2001). https://pubmed.ncbi.nlm.nih.gov/11420139/ DOI: 10.1016/s0304-4165(01)00130-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Aqueous assay; no tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7694fd3d-6797-52fa-baa1-cdeb764dfff0","evidence_kind":"source_excerpt","locator":"Lines 92-103","start_line":92,"end_line":103,"excerpt":"### boron-sam-binding\nS-adenosylmethionine formed a boron complex in capillary-electrophoresis experiments and ranked among the strongest ligands tested.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Boron can attach to SAM in a test tube; this does not show that it supplies methyl groups or improves methylation.\norganism: Cell-free chemistry\ntissue_or_cell_type: Aqueous assay; no tissue\nexperimental_model: Capillary electrophoresis of purified metabolites\nlimitations: Chemical affinity is not a demonstrated metabolic function or in-vivo occupancy. Rankings depend on assay and solution conditions.\nexposure: Boron complexation under the reported assay conditions\nevidence_span: {\"source_cache\": \"artifacts/boron-research/11420139.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0792ce09223d7cf23f0d3fda6b793e6e3b9904abe2c1f934b96d50e3a0e8b703\", \"start_char\": 0, \"end_char\": 1650, \"text_sha256\": \"0792ce09223d7cf23f0d3fda6b793e6e3b9904abe2c1f934b96d50e3a0e8b703\"}\n[boron-p11420139] Diadenosine phosphates and S-adenosylmethionine: novel boron binding biomolecules detected by capillary electrophoresis. (2001). https://pubmed.ncbi.nlm.nih.gov/11420139/ DOI: 10.1016/s0304-4165(01)00130-1","model_system":"Capillary electrophoresis of purified metabolites","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [boron-p11420139] Diadenosine phosphates and S-adenosylmethionine: novel boron binding biomolecules detected by capillary electrophoresis. (2001). https://pubmed.ncbi.nlm.nih.gov/11420139/ DOI: 10.1016/s0304-4165(01)00130-1","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2d96c547-aeec-5d8b-9d31-1288da209f89","stable_key":"import-7edf94bb-95c8-5234-9161-9eb338bb9b36","title":"Boron: chemistry, nutrient interactions, low-intake studies and mechanistic uncertainties (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"0a32c1c5c70cfb4a378f6ace35cf5b75d90ed3065ada91b9f9f64015d603c8e3","revision_id":"38f316f2-4344-542e-a638-0ac5402f678d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}