{"id":"be78dd4f-d71a-58aa-8b16-9b6e4d1241cd","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:protein-tml-release","predicate":"released_as","statement":"Proteolysis releases free trimethyllysine from trimethyllysine-containing protein, providing a precursor for carnitine biosynthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ca5d7fa3-a571-5156-a07a-2959bd5b44fe","mechanism_event_label":"Protein breakdown releases the modified lysine used to make carnitine.","subject":{"id":"9da7c88e-8ee8-5a05-bc08-737d580795b9","slug":"protein-bound-trimethyllysine","display_name":"Protein-bound N-epsilon-trimethyllysine residues","entity_type_key":"protein_state"},"object":{"id":"6ded5960-aad7-562a-95a1-ee6ad138d19e","slug":"trimethyllysine","display_name":"Free N-epsilon-trimethyl-L-lysine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ca5d7fa3-a571-5156-a07a-2959bd5b44fe","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:protein-tml-release-event","event_type":"biochemical_relationship","label":"Protein breakdown releases the modified lysine used to make carnitine.","description":"Proteolysis releases free trimethyllysine from trimethyllysine-containing protein, providing a precursor for carnitine biosynthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9da7c88e-8ee8-5a05-bc08-737d580795b9","slug":"protein-bound-trimethyllysine","display_name":"Protein-bound N-epsilon-trimethyllysine residues","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"316a3390-ac32-56e5-881e-fd9a521cce8f","slug":"lysosomal-proteolysis","display_name":"Lysosomal proteolysis","entity_type_key":"cellular_process"},"role":"process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ded5960-aad7-562a-95a1-ee6ad138d19e","slug":"trimethyllysine","display_name":"Free N-epsilon-trimethyl-L-lysine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Radiolabeled asialofetuin metabolism in rats and perfused rat liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Direct tracer evidence is rat; do not replace this with a direct free-lysine-to-carnitine edge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Protein breakdown releases the modified lysine used to make carnitine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[labadie1976] Hepatic synthesis of carnitine from protein-bound trimethyl-lysine. Lysosomal digestion of methyl-lysine-labelled asialo-fetuin (1976). https://pubmed.ncbi.nlm.nih.gov/64247/ DOI: 10.1042/bj1600085","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver lysosomes and downstream metabolism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"87780f9c-d4d9-5002-a548-c92654867b75","evidence_kind":"source_excerpt","locator":"Lines 156-164","start_line":156,"end_line":164,"excerpt":"### protein-tml-release\nProteolysis releases free trimethyllysine from trimethyllysine-containing protein, providing a precursor for carnitine biosynthesis.\nPlain language: Protein breakdown releases the modified lysine used to make carnitine.\nCondition category: normal\norganism: Rattus norvegicus\ntissue_or_cell_type: Liver lysosomes and downstream metabolism\nexperimental_model: Radiolabeled asialofetuin metabolism in rats and perfused rat liver\nlimitations: Direct tracer evidence is rat; do not replace this with a direct free-lysine-to-carnitine edge.\n[labadie1976] Hepatic synthesis of carnitine from protein-bound trimethyl-lysine. Lysosomal digestion of methyl-lysine-labelled asialo-fetuin (1976). https://pubmed.ncbi.nlm.nih.gov/64247/ DOI: 10.1042/bj1600085","model_system":"Radiolabeled asialofetuin metabolism in rats and perfused rat liver","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [labadie1976] Hepatic synthesis of carnitine from protein-bound trimethyl-lysine. Lysosomal digestion of methyl-lysine-labelled asialo-fetuin (1976). https://pubmed.ncbi.nlm.nih.gov/64247/ DOI: 10.1042/bj1600085","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}