{"id":"be5848ef-94bc-5f16-814c-ff20e637a413","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-binding-kainate","predicate":"competes_in_ligand_binding_assay_at","statement":"Theanine inhibited radioligand binding at kainate sites in rat cortical preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8deba414-5a21-58c8-899f-746d11ed48c8","mechanism_event_label":"A binding assay identifies a possible receptor interaction while leaving functional and clinical effects unresolved.","subject":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"object":{"id":"2be1ebbf-02c8-56bd-bfc5-b65c00538b43","slug":"rat-kainate-receptors","display_name":"Rat kainate-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"8deba414-5a21-58c8-899f-746d11ed48c8","stable_key":"8f12ba85-1a92-5337-a9c0-9cee9b3be90d:theanine-binding-kainate-event","event_type":"biochemical_relationship","label":"A binding assay identifies a possible receptor interaction while leaving functional and clinical effects unresolved.","description":"Theanine inhibited radioligand binding at kainate sites in rat cortical preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"33a051c5-dba8-53cd-8761-c53732c447e6","slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2be1ebbf-02c8-56bd-bfc5-b65c00538b43","slug":"rat-kainate-receptors","display_name":"Rat kainate-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/theanine-research/12596867.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"eaa387f7967a1ff8e68eceb71fd3294881967066c5dc080fd27bacf8a01ce33d\", \"start_char\": 0, \"end_char\": 643, \"text_sha256\": \"eaa387f7967a1ff8e68eceb71fd3294881967066c5dc080fd27bacf8a01ce33d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radioligand receptor-binding competition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Theanine competition with radiolabeled ligands","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding displacement is not proof of functional agonism/antagonism at an oral human dose. Abstract wording for the 80-30000-fold IC50 comparison is ambiguous and is not used as a potency estimate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Theanine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"theanine","display_name":"L-Theanine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat cortical preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A binding assay identifies a possible receptor interaction while leaving functional and clinical effects unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[theanine-p12596867] Inhibition by theanine of binding of [3H]AMPA, [3H]kainate, and [3H]MDL 105,519 to glutamate receptors. (2002). https://pubmed.ncbi.nlm.nih.gov/12596867/ DOI: 10.1271/bbb.66.2683","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"AMPA, kainate and NMDA glycine-site binding","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"16d05a64-38e4-5095-b997-88939f5de459","evidence_kind":"source_excerpt","locator":"Lines 302-313","start_line":302,"end_line":313,"excerpt":"### theanine-binding-kainate\nTheanine inhibited radioligand binding at kainate sites in rat cortical preparations.\nCondition category: normal\nnutrient_topic: L-Theanine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A binding assay identifies a possible receptor interaction while leaving functional and clinical effects unresolved.\norganism: Rat cortical preparations\ntissue_or_cell_type: AMPA, kainate and NMDA glycine-site binding\nexperimental_model: Radioligand receptor-binding competition\nlimitations: Binding displacement is not proof of functional agonism/antagonism at an oral human dose. Abstract wording for the 80-30000-fold IC50 comparison is ambiguous and is not used as a potency estimate.\nexposure: Theanine competition with radiolabeled ligands\nevidence_span: {\"source_cache\": \"artifacts/theanine-research/12596867.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"eaa387f7967a1ff8e68eceb71fd3294881967066c5dc080fd27bacf8a01ce33d\", \"start_char\": 0, \"end_char\": 643, \"text_sha256\": \"eaa387f7967a1ff8e68eceb71fd3294881967066c5dc080fd27bacf8a01ce33d\"}\n[theanine-p12596867] Inhibition by theanine of binding of [3H]AMPA, [3H]kainate, and [3H]MDL 105,519 to glutamate receptors. (2002). https://pubmed.ncbi.nlm.nih.gov/12596867/ DOI: 10.1271/bbb.66.2683","model_system":"Radioligand receptor-binding competition","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [theanine-p12596867] Inhibition by theanine of binding of [3H]AMPA, [3H]kainate, and [3H]MDL 105,519 to glutamate receptors. (2002). https://pubmed.ncbi.nlm.nih.gov/12596867/ DOI: 10.1271/bbb.66.2683","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2bf3fc8-fc9a-5d50-937a-559a76f8be7d","stable_key":"import-8f12ba85-1a92-5337-a9c0-9cee9b3be90d","title":"L-Theanine: metabolism, neural signaling, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"c78f2a1961da546cd8d3ae9131a16613aed0d1032904ca19175b0641647142c1","revision_id":"4ec64995-1e5b-5279-9102-f203cb2e29e5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}