{"id":"be3feae2-b35d-5ea6-b10e-e07dd4f6a928","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-erbeta-activation","predicate":"activates_transcription_without_direct_binding_via","statement":"DIM activated ERbeta target transcription without detectable binding to ERbeta in the study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ac5cf8d0-b415-5eb2-819d-4e93946527b2","mechanism_event_label":"Receptor-dependent signaling need not mean that DIM directly occupies the hormone-binding site.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"b393f98d-4dfc-508c-8812-7c2b6b1113b4","slug":"esr2","display_name":"Human estrogen receptor beta / ESR2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ac5cf8d0-b415-5eb2-819d-4e93946527b2","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-erbeta-activation-event","event_type":"biochemical_relationship","label":"Receptor-dependent signaling need not mean that DIM directly occupies the hormone-binding site.","description":"DIM activated ERbeta target transcription without detectable binding to ERbeta in the study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b393f98d-4dfc-508c-8812-7c2b6b1113b4","slug":"esr2","display_name":"Human estrogen receptor beta / ESR2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/20160136.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"558ee877330aede2675ccccdef5e31debf9824e00a1eaaacb1e7fe385d45b8e5\", \"start_char\": 0, \"end_char\": 1134, \"text_sha256\": \"558ee877330aede2675ccccdef5e31debf9824e00a1eaaacb1e7fe385d45b8e5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reporter, binding, chromatin and coactivator-silencing assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM and SRC-2 silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Subtype-selective response in this system; other studies find ERalpha responses under other hormonal and concentration conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human receptor-expressing cell systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Receptor-dependent signaling need not mean that DIM directly occupies the hormone-binding site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p20160136] Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane. (2010). https://pubmed.ncbi.nlm.nih.gov/20160136/ DOI: 10.1210/en.2009-1028","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ERbeta transcription","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"006058d3-915d-5095-99b5-4a3898b558b1","evidence_kind":"source_excerpt","locator":"Lines 571-582","start_line":571,"end_line":582,"excerpt":"### dim-erbeta-activation\nDIM activated ERbeta target transcription without detectable binding to ERbeta in the study.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Receptor-dependent signaling need not mean that DIM directly occupies the hormone-binding site.\norganism: Human receptor-expressing cell systems\ntissue_or_cell_type: ERbeta transcription\nexperimental_model: Reporter, binding, chromatin and coactivator-silencing assays\nlimitations: Subtype-selective response in this system; other studies find ERalpha responses under other hormonal and concentration conditions.\nexposure: DIM and SRC-2 silencing\nevidence_span: {\"source_cache\": \"artifacts/dim-research/20160136.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"558ee877330aede2675ccccdef5e31debf9824e00a1eaaacb1e7fe385d45b8e5\", \"start_char\": 0, \"end_char\": 1134, \"text_sha256\": \"558ee877330aede2675ccccdef5e31debf9824e00a1eaaacb1e7fe385d45b8e5\"}\n[dim-p20160136] Selective activation of estrogen receptor-beta target genes by 3,3'-diindolylmethane. 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