{"id":"be06f6b5-6fe6-5a5b-82db-319777b5366e","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:hcc-lysate-conversion","predicate":"reported_relationship","statement":"CrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e8255835-f99e-5b33-8785-746ae0fb3520","mechanism_event_label":"CrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.","subject":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"object":{"id":"253ef8c9-9c6c-50ce-9984-2cd131ccdefc","slug":"mouse-hcc-alcar-acetylcoa-formation","display_name":"Mouse HCC-lysate acetyl-CoA formation from ALCAR","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e8255835-f99e-5b33-8785-746ae0fb3520","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:hcc-lysate-conversion-event","event_type":"experimental_observation","label":"CrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.","description":"### hcc-lysate-conversion\n\nCrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.\nBoiled lysates failed to generate acetyl-CoA. This establishes residual catalytic capacity without identifying a universal cytosolic catalyst.\nModel and exposure: Wild-type, ACLY-null and ACLY/CrAT-null cell lysates; Fig. 7H.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"253ef8c9-9c6c-50ce-9984-2cd131ccdefc","slug":"mouse-hcc-alcar-acetylcoa-formation","display_name":"Mouse HCC-lysate acetyl-CoA formation from ALCAR","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"required reaction input","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"measured product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"224eacc1-fea4-5737-b661-56e7e41a64e7","slug":"mouse-crat","display_name":"Mouse carnitine acetyltransferase / Crat","entity_type_key":"protein"},"role":"deleted enzyme","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type, ACLY-null and ACLY/CrAT-null cell lysates; Fig. 7H.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"CrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference hcc-lysate-conversion; Boiled lysates failed to generate acetyl-CoA. This establishes residual catalytic capacity without identifying a universal cytosolic catalyst.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fcc3a273-0f1e-5de1-bfeb-ae3ca0c5b59d","evidence_kind":"source_excerpt","locator":"Lines 260-266","start_line":260,"end_line":266,"excerpt":"### hcc-lysate-conversion\n\nCrAT-null HCC-cell lysates retained enzyme-dependent conversion of ALCAR plus CoA to acetyl-CoA.\nBoiled lysates failed to generate acetyl-CoA. This establishes residual catalytic capacity without identifying a universal cytosolic catalyst.\nModel and exposure: Wild-type, ACLY-null and ACLY/CrAT-null cell lysates; Fig. 7H.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. | 2023 | https://pubmed.ncbi.nlm.nih.gov/37134161/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10156126/ | https://doi.org/10.1126/sciadv.adf0115 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Wild-type, ACLY-null and ACLY/CrAT-null cell lysates; Fig. 7H.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}