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This is distinct from cofactor-dependent gamma activation.\nexposure: 15 ng dialyzed PKC alpha; 45 µM FeCl2; 1 or 10 mM H2O2 activation for 2 min, stopped with 9 mM DTT; no PS or calcium cofactors.\ncross_nutrient: true\n[mccary2012] Vitamin E isoforms directly bind PKCα and differentially regulate activation of PKCα. (2012). https://pubmed.ncbi.nlm.nih.gov/21933153/ DOI: 10.1042/bj20111318","model_system":"Recombinant HIS-tagged human PKC alpha biochemical assay with iron and peroxide","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mccary2012] Vitamin E isoforms directly bind PKCα and differentially regulate activation of PKCα. 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