{"id":"bde03358-8db8-51b5-9a51-4bf973386945","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-gcn2-dispensability","predicate":"acts_without","statement":"Gcn2-null mice retained the methionine-restriction changes in adiposity, energy expenditure, insulin sensitivity and FGF21 induction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"95fb0795-1a86-5fdc-9141-934ec9a3817e","mechanism_event_label":"This dietary response did not require the usual amino-acid stress sensor.","subject":{"id":"2fa490e6-50cc-5a22-b1ea-af5e128666e4","slug":"experimental-methionine-restriction","display_name":"Experimentally methionine-restricted diet","entity_type_key":"cellular_process"},"object":{"id":"3876a24a-f6fe-5900-8ce2-22525a4faacb","slug":"mouse-eif2ak4","display_name":"Mouse GCN2 / Eif2ak4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"95fb0795-1a86-5fdc-9141-934ec9a3817e","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-gcn2-dispensability-event","event_type":"observed_relationship","label":"This dietary response did not require the usual amino-acid stress sensor.","description":"Gcn2-null mice retained the methionine-restriction changes in adiposity, energy expenditure, insulin sensitivity and FGF21 induction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2fa490e6-50cc-5a22-b1ea-af5e128666e4","slug":"experimental-methionine-restriction","display_name":"Experimentally methionine-restricted diet","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3876a24a-f6fe-5900-8ce2-22525a4faacb","slug":"mouse-eif2ak4","display_name":"Mouse GCN2 / Eif2ak4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8b01fe3c-a32b-5076-9c61-34d4d35e8b89","slug":"mouse-fgf21","display_name":"Mouse fibroblast growth factor 21 / Fgf21","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type and Gcn2-knockout mice under the study diet.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a universal statement about GCN2 during total starvation or other amino-acid deficiencies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"This dietary response did not require the usual amino-acid stress sensor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Role of GCN2-Independent Signaling Through a Noncanonical PERK/NRF2 Pathway in the Physiological Responses to Dietary Methionine Restriction. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26936965/ · DOI 10.2337/db15-1324","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"68aa930b-c150-5d80-9507-b28a619018c9","evidence_kind":"source_excerpt","locator":"Lines 380-386","start_line":380,"end_line":386,"excerpt":"## methionine-gcn2-dispensability\nThis dietary response did not require the usual amino-acid stress sensor.\nGcn2-null mice retained the methionine-restriction changes in adiposity, energy expenditure, insulin sensitivity and FGF21 induction.\nModel: Wild-type and Gcn2-knockout mice under the study diet.\nLimitations: Not a universal statement about GCN2 during total starvation or other amino-acid deficiencies.\nEvidence access: Primary abstract\nRole of GCN2-Independent Signaling Through a Noncanonical PERK/NRF2 Pathway in the Physiological Responses to Dietary Methionine Restriction. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26936965/ · DOI 10.2337/db15-1324","model_system":"Wild-type and Gcn2-knockout mice under the study diet.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f1c706ef72932eed85df234b6c3304158d503d626a63c69b02a453c162700d26","revision_id":"549035e0-3a72-550a-8a55-e8c5baa51b0d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}