{"id":"bd9df657-0335-5044-8dac-36ae68bcd920","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-halides","predicate":"does_not_reduce","statement":"Chloride did not act as electron donor; bromide reacted much more slowly, at (4.1 ± 0.1) × 10^4 M−1 s−1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f0774a7e-c473-5519-8d45-6fb774267737","mechanism_event_label":"Abundant chloride cannot simply replace iodide in this enzyme reaction.","subject":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"object":{"id":"0745f5ac-0f33-5adc-be03-c626ccb8f3ff","slug":"lpo-compound-i","display_name":"Lactoperoxidase compound I","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"f0774a7e-c473-5519-8d45-6fb774267737","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-halides-event","event_type":"observed_relationship","label":"Abundant chloride cannot simply replace iodide in this enzyme reaction.","description":"Chloride did not act as electron donor; bromide reacted much more slowly, at (4.1 ± 0.1) × 10^4 M−1 s−1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0745f5ac-0f33-5adc-be03-c626ccb8f3ff","slug":"lpo-compound-i","display_name":"Lactoperoxidase compound I","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"67cc3837-0c4f-5252-bb7e-c81992d11090","slug":"bromide-ion","display_name":"Bromide ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Study-specific exposure, including pharmacological and in-vitro conditions; normal is the schema fallback outside the three availability categories.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified enzyme kinetics; concentrations, substrate competition and reaction order determine relevance in tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium iodide research collection; shared-anion and comparator studies are not all KI interventions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium-iodide","display_name":"Potassium iodide","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Abundant chloride cannot simply replace iodide in this enzyme reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Reaction of lactoperoxidase compound I with halides and thiocyanate. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12269834/ · DOI 10.1021/bi026326x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c88d8043-6dd8-5897-b2e2-ac76a02e0e2e","evidence_kind":"source_excerpt","locator":"Lines 208-214","start_line":208,"end_line":214,"excerpt":"## ki-lpo-halides\nAbundant chloride cannot simply replace iodide in this enzyme reaction.\nChloride did not act as electron donor; bromide reacted much more slowly, at (4.1 ± 0.1) × 10^4 M−1 s−1.\nModel: Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.\nLimitations: Purified enzyme kinetics; concentrations, substrate competition and reaction order determine relevance in tissues.\nEvidence location: Primary abstract\nReaction of lactoperoxidase compound I with halides and thiocyanate. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12269834/ · DOI 10.1021/bi026326x","model_system":"Purified LPO transient kinetics; pH 7, 15 °C; preparation species unresolved in abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curation paraphrase; primary evidence location and source kind retained above.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc3ce411-f68f-5eb8-87c1-1592665f9cb6","stable_key":"import-79018983-9179-5c1a-8e7a-d6f47a13582b","title":"Potassium iodide: thyroid and non-thyroid mechanisms, interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-study references, chemical references and label statements individually identified. Not publisher full text.","file_path":"","sha256":"3ff9da818dd7a763a59c6abcff2eaee136d7f887a777034feaa5ba304a8fa6ce","revision_id":"53f2ae88-8a91-5326-ac49-3bb510ca78c3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}