{"id":"bd72377c-286e-5c9d-868b-40b0b730e3ee","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-iron-ii-binding","predicate":"binds_in_assay","statement":"Ferrozine competition supported SAC binding of Fe2+ in a chemical assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"05f60b73-7633-53cc-85fd-2e74633a1072","mechanism_event_label":"Iron binding is distinct from radical scavenging.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"05f60b73-7633-53cc-85fd-2e74633a1072","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-iron-ii-binding-event","event_type":"observed_relationship","label":"Iron binding is distinct from radical scavenging.","description":"Ferrozine competition supported SAC binding of Fe2+ in a chemical assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC in metal-binding and oxidative challenge assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ferrozine, electrochemistry and rat brain homogenate","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No binding constant or dietary iron-absorption effect was available from the accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Cell-free assays and rat brain preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Iron binding is distinct from radical scavenging.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[18422331] Antioxidant and iron-binding properties of curcumin, capsaicin, and S-allylcysteine reduce oxidative stress in rat brain homogenate. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18422331/ · DOI 10.1021/jf0734931","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Ferrozine, electrochemistry and rat brain homogenate","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d401a03-a6c6-5a46-b596-b9cf022b3328","evidence_kind":"source_excerpt","locator":"Lines 113-120","start_line":113,"end_line":120,"excerpt":"## s-allylcysteine-iron-ii-binding\nIron binding is distinct from radical scavenging.\nFerrozine competition supported SAC binding of Fe2+ in a chemical assay.\nModel: Ferrozine, electrochemistry and rat brain homogenate\nLimitations: No binding constant or dietary iron-absorption effect was available from the accessed abstract.\nEvidence access: Primary abstract\n[18422331] Antioxidant and iron-binding properties of curcumin, capsaicin, and S-allylcysteine reduce oxidative stress in rat brain homogenate. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18422331/ · DOI 10.1021/jf0734931\nStructured context: {\"organism\": \"Cell-free assays and rat brain preparation\", \"tissue_or_cell_type\": \"Ferrozine, electrochemistry and rat brain homogenate\", \"dose\": \"Not specified in accessed abstract\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"In vitro\", \"experimental_comparison\": \"SAC in metal-binding and oxidative challenge assays\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Ferrozine, electrochemistry and rat brain homogenate","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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