{"id":"bd70f485-81ce-55d7-8878-2919d17d91fc","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-ddo-methylation","predicate":"associates_with","statement":"Postnatal mouse Ddo promoter demethylation accompanied rising Ddo mRNA; azacitidine treatment increased Ddo transcripts in embryonic cortical neurons.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a8d171a3-2b16-5865-8bbc-5a31edb10aa8","mechanism_event_label":"Gene regulation can change the capacity to clear D-aspartate.","subject":{"id":"4e225692-c749-52b4-8d93-625c3642ed32","slug":"mouse-ddo-promoter-demethylation","display_name":"Mouse Ddo promoter demethylation","entity_type_key":"cellular_process"},"object":{"id":"f3fc3b2e-7748-5a0a-a936-94c47f68a6d2","slug":"mouse-ddo","display_name":"Mouse D-aspartate oxidase / Ddo","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a8d171a3-2b16-5865-8bbc-5a31edb10aa8","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-ddo-methylation-event","event_type":"observed_relationship","label":"Gene regulation can change the capacity to clear D-aspartate.","description":"Postnatal mouse Ddo promoter demethylation accompanied rising Ddo mRNA; azacitidine treatment increased Ddo transcripts in embryonic cortical neurons.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4e225692-c749-52b4-8d93-625c3642ed32","slug":"mouse-ddo-promoter-demethylation","display_name":"Mouse Ddo promoter demethylation","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f3fc3b2e-7748-5a0a-a936-94c47f68a6d2","slug":"mouse-ddo","display_name":"Mouse D-aspartate oxidase / Ddo","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse development and cultured embryonic-neuron demethylating-drug experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Azacitidine has broad effects. This is not evidence that folate intake directly switches DDO on or off.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Gene regulation can change the capacity to clear D-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Age-Related Changes in D-Aspartate Oxidase Promoter Methylation Control Extracellular D-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26961959/ · DOI 10.1523/JNEUROSCI.3881-15.2016","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6d13c6c8-441e-5aaf-97da-2ba8aaba0b98","evidence_kind":"source_excerpt","locator":"Lines 144-150","start_line":144,"end_line":150,"excerpt":"## d-aspartate-ddo-methylation\nGene regulation can change the capacity to clear D-aspartate.\nPostnatal mouse Ddo promoter demethylation accompanied rising Ddo mRNA; azacitidine treatment increased Ddo transcripts in embryonic cortical neurons.\nModel: Mouse development and cultured embryonic-neuron demethylating-drug experiments.\nLimitations: Azacitidine has broad effects. This is not evidence that folate intake directly switches DDO on or off.\nEvidence access: Primary abstract\nAge-Related Changes in D-Aspartate Oxidase Promoter Methylation Control Extracellular D-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26961959/ · DOI 10.1523/JNEUROSCI.3881-15.2016","model_system":"Mouse development and cultured embryonic-neuron demethylating-drug experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}