{"id":"bd1d9db5-84a0-56b7-83de-b6a3d88a20b9","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-rat-pyruvate-access","predicate":"inhibits","statement":"Fisetin inhibited pyruvate carboxylation in intact rat mitochondria, but not after freeze-thaw disruption.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"385cfc7d-4bcc-5441-82e8-b6b3225583a0","mechanism_event_label":"Membrane access may matter more than direct carboxylase inhibition.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"22f57b42-3477-53b8-b84b-540687c290ae","slug":"rat-intact-mitochondrial-pyruvate-carboxylation","display_name":"Pyruvate carboxylation in intact rat mitochondria","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"385cfc7d-4bcc-5441-82e8-b6b3225583a0","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-rat-pyruvate-access-event","event_type":"observed_relationship","label":"Membrane access may matter more than direct carboxylase inhibition.","description":"Fisetin inhibited pyruvate carboxylation in intact rat mitochondria, but not after freeze-thaw disruption.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"22f57b42-3477-53b8-b84b-540687c290ae","slug":"rat-intact-mitochondrial-pyruvate-carboxylation","display_name":"Pyruvate carboxylation in intact rat mitochondria","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Intact-mitochondrial IC50 approximately 163 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport limitation is an interpretation; no direct MPC binding or biotin antagonism was shown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Membrane access may matter more than direct carboxylase inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The actions of fisetin on glucose metabolism in the rat liver. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20084677/ · DOI 10.1002/cbf.1635","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d8b62457-635b-52d7-84fa-52e297d74f10","evidence_kind":"source_excerpt","locator":"Lines 328-334","start_line":328,"end_line":334,"excerpt":"## fisetin-rat-pyruvate-access\nMembrane access may matter more than direct carboxylase inhibition.\nFisetin inhibited pyruvate carboxylation in intact rat mitochondria, but not after freeze-thaw disruption.\nModel: Intact-mitochondrial IC50 approximately 163 micromolar.\nLimitations: Transport limitation is an interpretation; no direct MPC binding or biotin antagonism was shown.\nEvidence access: Primary abstract\nThe actions of fisetin on glucose metabolism in the rat liver. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20084677/ · DOI 10.1002/cbf.1635","model_system":"Intact-mitochondrial IC50 approximately 163 micromolar.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}