{"id":"bcfa1b7a-62c7-5f14-af08-846a8f7f2dbc","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-chicken-gldc-decarboxylation","predicate":"decarboxylates","statement":"Chicken P-protein alone catalyzed very slow glycine decarboxylation; added lipoic acid stimulated the measured decarboxylation at least 100-fold.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"75d5e46e-a654-5f31-a1e3-5bd232021c0d","mechanism_event_label":"B6-dependent glycine cleavage also depends on its lipoyl-linked reaction partners.","subject":{"id":"3ff7e3ba-6422-52ba-9f7b-acb263a4a47e","slug":"chicken-gldc","display_name":"Chicken glycine decarboxylase","entity_type_key":"protein"},"object":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"75d5e46e-a654-5f31-a1e3-5bd232021c0d","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-chicken-gldc-decarboxylation-event","event_type":"biochemical_relationship","label":"B6-dependent glycine cleavage also depends on its lipoyl-linked reaction partners.","description":"Chicken P-protein alone catalyzed very slow glycine decarboxylation; added lipoic acid stimulated the measured decarboxylation at least 100-fold.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"3ff7e3ba-6422-52ba-9f7b-acb263a4a47e","slug":"chicken-gldc","display_name":"Chicken glycine decarboxylase","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"966f14e5-c82f-5535-ad89-bfcc0adb2232","slug":"carbon-dioxide","display_name":"Carbon dioxide","entity_type_key":"small_molecule"},"role":"released product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"tested stimulator","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"PLP chemistry connects with the lipoyl component of glycine cleavage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified chicken liver mitochondrial P-protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Free lipoic acid assay; not evidence that supplements replace the intact cleavage system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Gallus gallus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"B6-dependent glycine cleavage also depends on its lipoyl-linked reaction partners.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Chicken liver mitochondrial protein","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"07c9c397-884e-52db-bcd8-a0344845b4bc","evidence_kind":"source_excerpt","locator":"Lines 659-669","start_line":659,"end_line":669,"excerpt":"### b6-met-chicken-gldc-decarboxylation\nChicken P-protein alone catalyzed very slow glycine decarboxylation; added lipoic acid stimulated the measured decarboxylation at least 100-fold.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: B6-dependent glycine cleavage also depends on its lipoyl-linked reaction partners.\norganism: Gallus gallus\ntissue_or_cell_type: Chicken liver mitochondrial protein\nexperimental_model: Purified chicken liver mitochondrial P-protein\nlimitations: Free lipoic acid assay; not evidence that supplements replace the intact cleavage system.\ncross_nutrient: PLP chemistry connects with the lipoyl component of glycine cleavage.\n[b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","model_system":"Purified chicken liver mitochondrial P-protein","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-gldc-1980] The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. (1980). https://pubmed.ncbi.nlm.nih.gov/7440562/ DOI: 10.1016/s0021-9258(19)70183-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}