{"id":"bcdf05e5-00dd-5d19-97d8-b40a1cb61e6c","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-er-calcium-storage","predicate":"reduces","statement":"Exposure to 50 µM boric acid for one hour reduced stored calcium by 32% in DU-145 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8c60f64a-0a98-54c4-be2c-a9a8a5c117b0","mechanism_event_label":"Less calcium remained in the measured internal stores after exposure.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"d56dc9ad-358a-50db-825d-47f04e38705e","slug":"er-calcium-storage","display_name":"Endoplasmic-reticulum calcium storage","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8c60f64a-0a98-54c4-be2c-a9a8a5c117b0","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-er-calcium-storage-event","event_type":"biochemical_relationship","label":"Less calcium remained in the measured internal stores after exposure.","description":"Exposure to 50 µM boric acid for one hour reduced stored calcium by 32% in DU-145 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d56dc9ad-358a-50db-825d-47f04e38705e","slug":"er-calcium-storage","display_name":"Endoplasmic-reticulum calcium storage","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Calcium imaging and flow cytometry in three human prostate cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"RyR-sensitive flux is not proof of direct binding to a particular RyR isoform. Cell-line exposure responses do not establish dietary prevention of prostate cancer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145, LNCaP and PWR1E cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Less calcium remained in the measured internal stores after exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. 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Cell-line exposure responses do not establish dietary prevention of prostate cancer.\nexposure: Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour\nevidence_span: {\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}\n[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. 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