{"id":"bcb5205c-afd2-56b2-98ca-14510a82fed3","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-bhmt-ko-sam","predicate":"deletion_decreases","statement":"Bhmt-null mouse liver contained 43% less SAM than wild type.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c8b222b7-e155-5f56-b253-614f33c959b0","mechanism_event_label":"The hepatic methyl-donor pool fell.","subject":{"id":"e6946c3a-4f7f-53b5-b813-762a6c5bf94c","slug":"bhmt-mouse-gene","display_name":"Bhmt gene (Mus musculus)","entity_type_key":"gene"},"object":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c8b222b7-e155-5f56-b253-614f33c959b0","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:folate-methyl-bhmt-ko-sam-event","event_type":"biochemical_relationship","label":"The hepatic methyl-donor pool fell.","description":"Bhmt-null mouse liver contained 43% less SAM than wild type.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e6946c3a-4f7f-53b5-b813-762a6c5bf94c","slug":"bhmt-mouse-gene","display_name":"Bhmt gene (Mus musculus)","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Bhmt-null mice and wild-type controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Concentration is not methylation flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The hepatic methyl-donor pool fell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[teng-2011] Deletion of betaine-homocysteine S-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in fatty liver and hepatocellular carcinomas (2011). https://pubmed.ncbi.nlm.nih.gov/21878621/ DOI: 10.1074/jbc.m111.265348","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"0367cfe7-2b45-5d75-95fc-e1d4e9ac657f","evidence_kind":"source_excerpt","locator":"Lines 646-655","start_line":646,"end_line":655,"excerpt":"### folate-methyl-bhmt-ko-sam\nBhmt-null mouse liver contained 43% less SAM than wild type.\nCondition category: machinery_impairment\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The hepatic methyl-donor pool fell.\norganism: Mus musculus\ntissue_or_cell_type: Liver\nexperimental_model: Bhmt-null mice and wild-type controls.\nlimitations: Concentration is not methylation flux.\n[teng-2011] Deletion of betaine-homocysteine S-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in fatty liver and hepatocellular carcinomas (2011). https://pubmed.ncbi.nlm.nih.gov/21878621/ DOI: 10.1074/jbc.m111.265348","model_system":"Bhmt-null mice and wild-type controls.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [teng-2011] Deletion of betaine-homocysteine S-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in fatty liver and hepatocellular carcinomas (2011). https://pubmed.ncbi.nlm.nih.gov/21878621/ DOI: 10.1074/jbc.m111.265348","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}