{"id":"bc7a2d16-7601-5f9f-b7c3-371ff1d40734","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-are-reporter","predicate":"increased","statement":"Lycopene treatment increased ARE-driven reporter expression in transfected cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2d9c2fb1-f812-5c8e-b374-3490ca60d6b1","mechanism_event_label":"Cells activated a protective gene program in this experiment.","subject":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"object":{"id":"4d1a3f06-1ac6-591f-935c-7277165c556b","slug":"are-reporter-transcription","display_name":"Antioxidant response element reporter transcription","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2d9c2fb1-f812-5c8e-b374-3490ca60d6b1","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-are-reporter-event","event_type":"biochemical_relationship","label":"Cells activated a protective gene program in this experiment.","description":"Lycopene treatment increased ARE-driven reporter expression in transfected cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d1a3f06-1ac6-591f-935c-7277165c556b","slug":"are-reporter-transcription","display_name":"Antioxidant response element reporter transcription","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/15657364.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\", \"start_char\": 0, \"end_char\": 1890, \"text_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reporter, expression and dominant-negative transcription-factor experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lycopene and ethanolic derivative extract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Unidentified derivative extract was active; direct binding of intact lycopene to KEAP1 was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cancer cell cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cells activated a protective gene program in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p15657364] Carotenoids activate the antioxidant response element transcription system. (2005). https://pubmed.ncbi.nlm.nih.gov/15657364/ DOI: 10.1158/1535-7163.177.4.1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ARE transcription and phase II enzyme expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"81a7fa41-0f92-59ef-b2fd-7fe00635f635","evidence_kind":"source_excerpt","locator":"Lines 442-453","start_line":442,"end_line":453,"excerpt":"### lycopene-are-reporter\nLycopene treatment increased ARE-driven reporter expression in transfected cancer cells.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Cells activated a protective gene program in this experiment.\norganism: Human cancer cell cultures\ntissue_or_cell_type: ARE transcription and phase II enzyme expression\nexperimental_model: Reporter, expression and dominant-negative transcription-factor experiments\nlimitations: Unidentified derivative extract was active; direct binding of intact lycopene to KEAP1 was not established.\nexposure: Lycopene and ethanolic derivative extract\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/15657364.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\", \"start_char\": 0, \"end_char\": 1890, \"text_sha256\": \"a25b93d6074a1e560bf1e163aa70c511e99199b9bb62ccdffdbe70e51fcc7c32\"}\n[lycopene-p15657364] Carotenoids activate the antioxidant response element transcription system. 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