{"id":"bc6a47c4-82ea-5288-afcb-1fb41578cf7e","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-rat-epimerase-low","predicate":"reduced_in","statement":"Myo-to-chiro epimerase activity was lower in liver, muscle and fat cytosol from diabetic GK rats than Wistar controls.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"095a0415-87b2-5bc8-a808-e161767a3426","mechanism_event_label":"Reduced conversion accompanied insulin resistance in these rat tissues; causation was not settled.","subject":{"id":"65abfe35-d1a6-568c-baee-2d929a2a2ec0","slug":"rat-myo-dci-epimerase-activity","display_name":"Myo-to-D-chiro-inositol epimerase activity in rat cytosol","entity_type_key":"cellular_process"},"object":{"id":"125c5583-8d1a-5662-92bc-26ce71a6856e","slug":"gk-rat-diabetic-state","display_name":"Diabetic state in Goto-Kakizaki rats","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"095a0415-87b2-5bc8-a808-e161767a3426","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-rat-epimerase-low-event","event_type":"observed_intervention","label":"Reduced conversion accompanied insulin resistance in these rat tissues; causation was not settled.","description":"Myo-to-chiro epimerase activity was lower in liver, muscle and fat cytosol from diabetic GK rats than Wistar controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"65abfe35-d1a6-568c-baee-2d929a2a2ec0","slug":"rat-myo-dci-epimerase-activity","display_name":"Myo-to-D-chiro-inositol epimerase activity in rat cytosol","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"125c5583-8d1a-5662-92bc-26ce71a6856e","slug":"gk-rat-diabetic-state","display_name":"Diabetic state in Goto-Kakizaki rats","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/12051772.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28ae1225b489898738997d2376ab09857f017314086979032d3ad907299a1154\", \"start_char\": 0, \"end_char\": 2218, \"text_sha256\": \"28ae1225b489898738997d2376ab09857f017314086979032d3ad907299a1154\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiotracer conversion and cytosolic enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Diabetic rat versus controls; reduced pyridine-nucleotide cofactors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The molecular identity of the activity was not established here. A later mouse stable-isotope study found no myo-to-D-chiro conversion; the research disagreement is recorded separately. Do not invent a human epimerase gene or infer a universal supplement ratio.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Goto-Kakizaki and Wistar rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reduced conversion accompanied insulin resistance in these rat tissues; causation was not settled.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p12051772] Both myo-inositol to chiro-inositol epimerase activities and chiro-inositol to myo-inositol ratios are decreased in tissues of GK type 2 diabetic rats compared to Wistar controls. (2002). https://pubmed.ncbi.nlm.nih.gov/12051772/ DOI: 10.1016/s0006-291x(02)00313-3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver, muscle, kidney and adipose preparations","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"97a1eae1-49fb-5e90-b182-742fa3499720","evidence_kind":"source_excerpt","locator":"Lines 1198-1209","start_line":1198,"end_line":1209,"excerpt":"### ino-rat-epimerase-low\nMyo-to-chiro epimerase activity was lower in liver, muscle and fat cytosol from diabetic GK rats than Wistar controls.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reduced conversion accompanied insulin resistance in these rat tissues; causation was not settled.\norganism: Goto-Kakizaki and Wistar rats\ntissue_or_cell_type: Liver, muscle, kidney and adipose preparations\nexperimental_model: Radiotracer conversion and cytosolic enzyme assays\nlimitations: The molecular identity of the activity was not established here. A later mouse stable-isotope study found no myo-to-D-chiro conversion; the research disagreement is recorded separately. Do not invent a human epimerase gene or infer a universal supplement ratio.\nexposure: Diabetic rat versus controls; reduced pyridine-nucleotide cofactors\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/12051772.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"28ae1225b489898738997d2376ab09857f017314086979032d3ad907299a1154\", \"start_char\": 0, \"end_char\": 2218, \"text_sha256\": \"28ae1225b489898738997d2376ab09857f017314086979032d3ad907299a1154\"}\n[ino-p12051772] Both myo-inositol to chiro-inositol epimerase activities and chiro-inositol to myo-inositol ratios are decreased in tissues of GK type 2 diabetic rats compared to Wistar controls. (2002). https://pubmed.ncbi.nlm.nih.gov/12051772/ DOI: 10.1016/s0006-291x(02)00313-3","model_system":"Radiotracer conversion and cytosolic enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p12051772] Both myo-inositol to chiro-inositol epimerase activities and chiro-inositol to myo-inositol ratios are decreased in tissues of GK type 2 diabetic rats compared to Wistar controls. (2002). https://pubmed.ncbi.nlm.nih.gov/12051772/ DOI: 10.1016/s0006-291x(02)00313-3","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}