{"id":"bc540366-1e97-50bb-b6af-1071e54d8a4e","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-ampk-context","predicate":"not_required_in_study","statement":"Autophagy remained unimpaired in AMPK-deficient cells during amino-acid deprivation; ULK1 signaling and LC3B lipidation increased.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e3cec134-991b-59c1-babf-7491291b9649","mechanism_event_label":"Loss of this sensor did not shut down all autophagy.","subject":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"object":{"id":"8f3e8b4e-812d-5a5c-8b34-ebfd4cfd0d26","slug":"cellular-autophagic-flux","display_name":"Cellular autophagic flux, experimental context specified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e3cec134-991b-59c1-babf-7491291b9649","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-ampk-context-event","event_type":"observed_relationship","label":"Loss of this sensor did not shut down all autophagy.","description":"Autophagy remained unimpaired in AMPK-deficient cells during amino-acid deprivation; ULK1 signaling and LC3B lipidation increased.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f3e8b4e-812d-5a5c-8b34-ebfd4cfd0d26","slug":"cellular-autophagic-flux","display_name":"Cellular autophagic flux, experimental context specified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"78a748c5-cbd6-5b85-89e9-d60e9f59bebb","slug":"experimental-amino-acid-deprivation","display_name":"Experimental amino-acid deprivation","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Several cultured cell lines under prolonged amino-acid withdrawal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different stress from glucose withdrawal; a universal AMPK-on/autophagy-on rule is unsupported.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"Loss of this sensor did not shut down all autophagy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Unexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38744665/ · DOI 10.1080/15548627.2024.2355074","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7d02de8b-6335-535b-9f6f-035c97afbc59","evidence_kind":"source_excerpt","locator":"Lines 312-318","start_line":312,"end_line":318,"excerpt":"## fast-ampk-context\nLoss of this sensor did not shut down all autophagy.\nAutophagy remained unimpaired in AMPK-deficient cells during amino-acid deprivation; ULK1 signaling and LC3B lipidation increased.\nModel: Several cultured cell lines under prolonged amino-acid withdrawal.\nLimitations: Different stress from glucose withdrawal; a universal AMPK-on/autophagy-on rule is unsupported.\nEvidence access: Primary abstract\nUnexpected roles for AMPK in the suppression of autophagy and the reactivation of MTORC1 signaling during prolonged amino acid deprivation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38744665/ · DOI 10.1080/15548627.2024.2355074","model_system":"Several cultured cell lines under prolonged amino-acid withdrawal.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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