{"id":"bbdc4700-f001-511d-a954-41d0f801a9a6","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-antioxidant-markers","predicate":"changes","statement":"A 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e2e246f1-dd5b-5c20-a788-9129d6ec6a00","mechanism_event_label":"A 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.","subject":{"id":"45a7aaec-8acc-5d5a-a014-c483ef5385e2","slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"},"object":{"id":"8200c3d6-b155-5919-859a-69ef6e753825","slug":"human-antioxidant-and-oxidation-marker-profile","display_name":"Human antioxidant and oxidation marker profile","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e2e246f1-dd5b-5c20-a788-9129d6ec6a00","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-antioxidant-markers-event","event_type":"observed_relationship","label":"A 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.","description":"A 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"45a7aaec-8acc-5d5a-a014-c483ef5385e2","slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8200c3d6-b155-5919-859a-69ef6e753825","slug":"human-antioxidant-and-oxidation-marker-profile","display_name":"Human antioxidant and oxidation marker profile","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"15 mg/day hydroxytyrosol formulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"3 weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Healthy human volunteers in a randomized double-blind crossover trial","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small surrogate-marker study; body-composition changes were small and do not establish disease prevention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hydroxytyrosol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Healthy human volunteers in a randomized double-blind crossover trial","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial. (2017). https://pubmed.ncbi.nlm.nih.gov/28855976/ DOI: 10.1155/2017/2473495","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Oral gastroresistant capsules","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Serum metabolites, oxidation markers and gene expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9635487e-cc73-5317-9c65-5129222a2422","evidence_kind":"source_excerpt","locator":"Lines 88-97","start_line":88,"end_line":97,"excerpt":"## hydroxytyrosol-antioxidant-markers\nA 15 mg/day gastroresistant formulation for three weeks increased thiol groups, total antioxidant status and SOD1 expression while reducing nitrite, nitrate and malondialdehyde in healthy volunteers.\nModel/species: Healthy human volunteers in a randomized double-blind crossover trial\nTissue/system: Serum metabolites, oxidation markers and gene expression\nExposure: 15 mg/day hydroxytyrosol formulation\nRoute: Oral gastroresistant capsules\nDuration: 3 weeks\nLimits: Small surrogate-marker study; body-composition changes were small and do not establish disease prevention.\nPrimary reference: Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial. (2017). https://pubmed.ncbi.nlm.nih.gov/28855976/ DOI: 10.1155/2017/2473495\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Healthy human volunteers in a randomized double-blind crossover trial","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f83cd982-34f0-55d4-8563-eedfb0f009f2","stable_key":"import-cb41aa31-e26b-5b60-b7e0-e4985fb7aea8","title":"Hydroxytyrosol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"7677fedffc16af238fc1ace3f88d206dd42b29b163c40b6e69ba6ed2457a3aee","revision_id":"1e0bd617-6834-5a2d-a74e-c058967d99b7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}