{"id":"bbb7b92e-393e-5046-8b6a-86042cc2d27f","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-rat-leydig","predicate":"increases_tested","statement":"Rat Leydig cells incubated with 0.1 or 1 mM D-aspartate showed increased measured testosterone and cAMP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a83e40cc-78f5-5475-9faa-8df85e983766","mechanism_event_label":"A second rat experiment located a response in steroid-producing cells.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"2016e86d-92c9-519e-996a-4579d90d7414","slug":"rat-leydig-testosterone-output","display_name":"Testosterone output in rat Leydig-cell incubation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a83e40cc-78f5-5475-9faa-8df85e983766","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-rat-leydig-event","event_type":"observed_relationship","label":"A second rat experiment located a response in steroid-producing cells.","description":"Rat Leydig cells incubated with 0.1 or 1 mM D-aspartate showed increased measured testosterone and cAMP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2016e86d-92c9-519e-996a-4579d90d7414","slug":"rat-leydig-testosterone-output","display_name":"Testosterone output in rat Leydig-cell incubation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e3013e06-20da-5c91-b8f8-d9364a645fae","slug":"testosterone","display_name":"Testosterone","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9cc3323e-3a7f-5be5-91ae-78dab284fb81","slug":"cyclic-amp","display_name":"Cyclic adenosine monophosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat Leydig cells, 60-minute incubations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"cAMP involvement is supported by co-change, not an exclusive pathway demonstrated by blocking or rescue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; 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