{"id":"bb9cd148-f3b3-50f5-aecb-09255fde3bf1","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-open-lobe","predicate":"retains","statement":"The vanadium adduct retained an open N-lobe and closed iron-containing C-lobe with little overall structural change.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6bf4faae-a860-5c18-ba06-a6d3a84fa3d4","mechanism_event_label":"A binding structure alone does not demonstrate successful cellular delivery.","subject":{"id":"1b146e35-4788-54cf-b511-185b4b6736bc","slug":"human-transferrin-tyr188-divanadate-adduct","display_name":"Human C-lobe-ferric transferrin Tyr188-linked divanadate(V) adduct","entity_type_key":"protein_state"},"object":{"id":"4cce242a-44be-59ea-a896-1ab84b3cd623","slug":"human-transferrin-open-n-lobe","display_name":"Open N-lobe conformation of monoferric human transferrin","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6bf4faae-a860-5c18-ba06-a6d3a84fa3d4","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-tf-open-lobe-event","event_type":"observed_relationship","label":"A binding structure alone does not demonstrate successful cellular delivery.","description":"The vanadium adduct retained an open N-lobe and closed iron-containing C-lobe with little overall structural change.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1b146e35-4788-54cf-b511-185b4b6736bc","slug":"human-transferrin-tyr188-divanadate-adduct","display_name":"Human C-lobe-ferric transferrin Tyr188-linked divanadate(V) adduct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4cce242a-44be-59ea-a896-1ab84b3cd623","slug":"human-transferrin-open-n-lobe","display_name":"Open N-lobe conformation of monoferric human transferrin","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ac656bc2-967c-568b-b6d5-58c7ff433c94","slug":"tf","display_name":"Human serum transferrin / TF","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"05415b89-a435-5940-a9ba-b587a0b6f04d","slug":"tfrc","display_name":"Human transferrin receptor 1 / TFRC","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transferrin crystal comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Compatibility with receptor recognition is a structural interpretation, not a completed cellular uptake cycle; not a contradiction of the A549 transport study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A binding structure alone does not demonstrate successful cellular delivery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"First crystal structure of an adduct formed upon reaction of a vanadium compound with human serum transferrin. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41545537/ · DOI 10.1038/s42004-026-01891-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"28584fb5-d780-5c48-bac5-3f4e6fbd4f51","evidence_kind":"source_excerpt","locator":"Lines 222-228","start_line":222,"end_line":228,"excerpt":"## vanadium-tf-open-lobe\nA binding structure alone does not demonstrate successful cellular delivery.\nThe vanadium adduct retained an open N-lobe and closed iron-containing C-lobe with little overall structural change.\nModel: Human transferrin crystal comparison.\nLimitations: Compatibility with receptor recognition is a structural interpretation, not a completed cellular uptake cycle; not a contradiction of the A549 transport study.\nEvidence access: Primary full text\nFirst crystal structure of an adduct formed upon reaction of a vanadium compound with human serum transferrin. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41545537/ · DOI 10.1038/s42004-026-01891-1","model_system":"Human transferrin crystal comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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