{"id":"bb42a5dc-3c58-58be-b774-f63eae92afb1","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-gsdmd","predicate":"decreases_in_recorded_comparison","statement":"Apigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"468eb912-b49d-5f89-8722-d84a50d7eb1b","mechanism_event_label":"Apigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"a29f5438-78e3-5f8e-b037-94316ef8ecb4","slug":"gsdmd","display_name":"GSDMD","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"468eb912-b49d-5f89-8722-d84a50d7eb1b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-human-gsdmd-event","event_type":"experimental_observation","label":"Apigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.","description":"### api-human-gsdmd\n\nApigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.\nThis cleavage readout does not establish direct binding to gasdermin D.\nModel and exposure: Figure 3M–N; 100 micromolar apigenin; ATP 3 mM for 15 minutes.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin plus LPS/ATP","sequence_order":0,"notes":""},{"entity":{"id":"a29f5438-78e3-5f8e-b037-94316ef8ecb4","slug":"gsdmd","display_name":"GSDMD","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"extracellular activation signal","comparator":"LPS/ATP alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"Apigenin plus LPS/ATP","comparator":"LPS/ATP alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"prior priming","comparator":"LPS/ATP alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin plus LPS/ATP\", \"comparator\": \"LPS/ATP alone\", \"endpoint\": \"Apigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin plus LPS/ATP\"}, {\"entity_slug\": \"lipopolysaccharide\", \"state\": \"prior priming\"}, {\"entity_slug\": \"atp\", \"state\": \"extracellular activation signal\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Figure 3M–N; 100 micromolar apigenin; ATP 3 mM for 15 minutes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-human-gsdmd; This cleavage readout does not establish direct binding to gasdermin D.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f8f80fb3-1bf6-5bf4-82c5-511ab44a1fcf","evidence_kind":"source_excerpt","locator":"Lines 364-370","start_line":364,"end_line":370,"excerpt":"### api-human-gsdmd\n\nApigenin reduced ATP-induced N-terminal gasdermin-D formation in LPS-primed human monocytes.\nThis cleavage readout does not establish direct binding to gasdermin D.\nModel and exposure: Figure 3M–N; 100 micromolar apigenin; ATP 3 mM for 15 minutes.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin inhibits NLRP3 inflammasome activation in monocytes and macrophages independently of CD38. | 2024 | https://pubmed.ncbi.nlm.nih.gov/39840045/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11746122/ | https://doi.org/10.3389/fimmu.2024.1497984 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Figure 3M–N; 100 micromolar apigenin; ATP 3 mM for 15 minutes.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}