{"id":"bb3cab5c-6b63-5044-9a79-c802621c0bc9","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-lowers-mitochondrial-membrane-potential","predicate":"reduces","statement":"Lariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"f2d1b36f-15de-525b-ab14-8adb213e2b47","mechanism_event_label":"Lariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.","subject":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"object":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f2d1b36f-15de-525b-ab14-8adb213e2b47","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-lowers-mitochondrial-membrane-potential-event","event_type":"observed_relationship","label":"Lariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.","description":"Lariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f9332e0-0138-5e42-8c7b-8f8f45615782","slug":"mitochondrial-membrane-potential","display_name":"Inner mitochondrial membrane potential","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"346790f2-c292-5dcf-8266-2332c41c7c38","slug":"casp3","display_name":"Human caspase-3 / CASP3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HepG2 hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lariciresinol, dose-dependent","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The enantiomer is not stated, and the abstract does not give the concentration range or the inhibitory concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human HepG2 hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lariciresinol induces apoptosis in HepG2 cells via mitochondrial-mediated apoptosis pathway. (2018). https://pubmed.ncbi.nlm.nih.gov/29247613/ DOI: 10.1016/j.ejphar.2017.12.015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Mitochondrial membrane potential and apoptosis-associated proteins","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"131f8194-390e-55a2-beba-c6c478867996","evidence_kind":"source_excerpt","locator":"Lines 209-218","start_line":209,"end_line":218,"excerpt":"## lariciresinol-lowers-mitochondrial-membrane-potential\nLariciresinol lowered mitochondrial membrane potential in hepatoma cells, measured by JC-1 staining, alongside apoptosis-associated protein changes on western blot.\nModel/species: Human HepG2 hepatoma cells\nTissue/system: Mitochondrial membrane potential and apoptosis-associated proteins\nExposure: Lariciresinol, dose-dependent\nRoute: In vitro\nDuration: Not stated here\nLimits: The enantiomer is not stated, and the abstract does not give the concentration range or the inhibitory concentration.\nPrimary reference: Lariciresinol induces apoptosis in HepG2 cells via mitochondrial-mediated apoptosis pathway. (2018). https://pubmed.ncbi.nlm.nih.gov/29247613/ DOI: 10.1016/j.ejphar.2017.12.015\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}