{"id":"bb375e0a-9922-5641-87cc-e383a55d50cd","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-colgalt-gt2-metal","predicate":"binds_catalytic_domain_of","statement":"The COLGALT1 GT2 catalytic site contains a Glu-Asp-Asp motif important for manganese binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"529b309d-89d7-596c-ac3b-4c946ec5892e","mechanism_event_label":"The collagen sugar-transfer reaction has a defined manganese-binding site.","subject":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"object":{"id":"4dbb65a6-60cb-5fad-b3eb-73ee7038381d","slug":"colgalt1","display_name":"COLGALT1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"529b309d-89d7-596c-ac3b-4c946ec5892e","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-gly-colgalt-gt2-metal-event","event_type":"biochemical_relationship","label":"The collagen sugar-transfer reaction has a defined manganese-binding site.","description":"The COLGALT1 GT2 catalytic site contains a Glu-Asp-Asp motif important for manganese binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a76bbd5b-08c9-5d74-8b6f-d69502ffa655","slug":"udp-galactose","display_name":"UDP-galactose","entity_type_key":"small_molecule"},"role":"donor_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4dbb65a6-60cb-5fad-b3eb-73ee7038381d","slug":"colgalt1","display_name":"COLGALT1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"UDP-galactose (donor_substrate)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/manganese-glycosylation-sources/colgalt2025.abstract.txt\", \"locator\": \"Indexed primary abstract\", \"file_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"start_char\": 0, \"end_char\": 1331, \"text_sha256\": \"5a42e5928c56f4e312768d79c9a5601b6f9e76a44bcdd9ede4bc8ea0c8985b60\", \"text_characters\": 1331}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human COLGALT1 structural and biochemical assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Metal substitution and structural-domain comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Modification of collagen-bound hydroxylysine does not establish that extra dietary manganese or free lysine improves collagen in a person.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The collagen sugar-transfer reaction has a defined manganese-binding site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-gly-colgalt2025] Molecular structure and enzymatic mechanism of the human collagen hydroxylysine galactosyltransferase GLT25D1/COLGALT1. 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