{"id":"baf58832-a0d1-5853-8e5e-5f132c5d3f95","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-liver-atf4","predicate":"induces","statement":"The study linked ATF4-dependent stress signaling to increased hepatic Gpt2 expression in the examined mouse ER-stress and obesity settings.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e300fabe-8881-5a8f-a35d-4cd652aba021","mechanism_event_label":"Stress can change how much alanine-processing enzyme a liver expresses.","subject":{"id":"7a0fa32a-a00f-5500-bce7-8e8d101a8e2b","slug":"mouse-atf4","display_name":"Mouse activating transcription factor 4 / Atf4","entity_type_key":"protein"},"object":{"id":"f78af6f3-4504-551f-b48b-dd25fcf5a629","slug":"mouse-gpt2","display_name":"Mouse mitochondrial alanine aminotransferase / Gpt2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e300fabe-8881-5a8f-a35d-4cd652aba021","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-liver-atf4-event","event_type":"observed_relationship","label":"Stress can change how much alanine-processing enzyme a liver expresses.","description":"The study linked ATF4-dependent stress signaling to increased hepatic Gpt2 expression in the examined mouse ER-stress and obesity settings.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7a0fa32a-a00f-5500-bce7-8e8d101a8e2b","slug":"mouse-atf4","display_name":"Mouse activating transcription factor 4 / Atf4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f78af6f3-4504-551f-b48b-dd25fcf5a629","slug":"mouse-gpt2","display_name":"Mouse mitochondrial alanine aminotransferase / Gpt2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse hepatocyte/liver interventions and expression assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression regulation is context-specific and does not establish a human supplementation response. Correction record: Published correction adds the originally omitted disclosure that B.N.F. was a scientific advisory board member and stockholder of Cirius Therapeutics, which develops MPC inhibitors. Disclosure correction, not a data retraction. https://pmc.ncbi.nlm.nih.gov/articles/PMC9713851/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Stress can change how much alanine-processing enzyme a liver expresses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Silencing alanine transaminase 2 in diabetic liver attenuates hyperglycemia by reducing gluconeogenesis from amino acids. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35476997/ · DOI 10.1016/j.celrep.2022.110733","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e90d34e6-4b31-52c7-9779-bad4a0276c47","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"## alanine-liver-atf4\nStress can change how much alanine-processing enzyme a liver expresses.\nThe study linked ATF4-dependent stress signaling to increased hepatic Gpt2 expression in the examined mouse ER-stress and obesity settings.\nModel: Mouse hepatocyte/liver interventions and expression assays.\nLimitations: Expression regulation is context-specific and does not establish a human supplementation response. Correction record: Published correction adds the originally omitted disclosure that B.N.F. was a scientific advisory board member and stockholder of Cirius Therapeutics, which develops MPC inhibitors. Disclosure correction, not a data retraction. https://pmc.ncbi.nlm.nih.gov/articles/PMC9713851/\nEvidence access: Primary full text\nSilencing alanine transaminase 2 in diabetic liver attenuates hyperglycemia by reducing gluconeogenesis from amino acids. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35476997/ · DOI 10.1016/j.celrep.2022.110733","model_system":"Mouse hepatocyte/liver interventions and expression assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8ce7dd76c88b2e25fa23d93f9655a244aadb9d90f55ec8b18ecf0fd4625a61d2","revision_id":"4ed32258-0213-599a-a272-6f8f62280773","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}