{"id":"bae62544-e419-5de6-ac98-ac89ea338328","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-nitration","predicate":"forms_nitrated_derivatives","statement":"CLA yielded substantially more nitration products than nonconjugated linoleic acid under the tested nitrogen-oxide reactions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f6505f52-cb32-59c3-9533-8d0cc6afdb23","mechanism_event_label":"Its double-bond arrangement changes its chemical reactivity.","subject":{"id":"7805c8e6-fae3-509c-b152-4d3c3ed0d5d3","slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"},"object":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"f6505f52-cb32-59c3-9533-8d0cc6afdb23","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-nitration-event","event_type":"observed_relationship","label":"Its double-bond arrangement changes its chemical reactivity.","description":"CLA yielded substantially more nitration products than nonconjugated linoleic acid under the tested nitrogen-oxide reactions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7805c8e6-fae3-509c-b152-4d3c3ed0d5d3","slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"806c5846-1ca4-5854-b68d-699af6b447fb","slug":"nitro-conjugated-linoleic-acid","display_name":"Nitro-conjugated linoleic acid / NO2-CLA family","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"03ffed32-aa18-5d63-96a4-8a7ebedaf7a9","slug":"nitrite","display_name":"Nitrite / NO2(-)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8744fb0d-739e-52cc-ac18-2f00ac889720","slug":"linoleic-acid","display_name":"Linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chemical, cellular and in-vivo nitration experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is formation of a different molecule, not evidence that plain CLA has every nitro-lipid action.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Its double-bond arrangement changes its chemical reactivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Conjugated linoleic acid is a preferential substrate for fatty acid nitration. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23144452/ · DOI 10.1074/jbc.M112.401356","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"99a00aa8-c38b-5304-a6ec-1898efb1572a","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## cla-nitration\nIts double-bond arrangement changes its chemical reactivity.\nCLA yielded substantially more nitration products than nonconjugated linoleic acid under the tested nitrogen-oxide reactions.\nModel: Chemical, cellular and in-vivo nitration experiments.\nLimitations: This is formation of a different molecule, not evidence that plain CLA has every nitro-lipid action.\nEvidence access: Primary abstract\nConjugated linoleic acid is a preferential substrate for fatty acid nitration. · 2012 · https://pubmed.ncbi.nlm.nih.gov/23144452/ · DOI 10.1074/jbc.M112.401356","model_system":"Chemical, cellular and in-vivo nitration experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}