{"id":"bad6592c-a8bc-5c78-8f1c-1efbe3afa545","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-mitoros-rescue","predicate":"attenuates_tested_inhibition_of","statement":"Mito-TEMPO and NAC prevented tested cucurbitacin I effects on Rac1 activation, supporting mitochondrial ROS involvement alongside the thiol-interception caveat for NAC.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e481a78f-cbe1-5a09-9a04-6d2c1cc651c1","mechanism_event_label":"Two different probes help test the oxidative-stress route.","subject":{"id":"26bd3a3e-663f-5af9-8427-cf8ddc1f6f25","slug":"mitotempo","display_name":"Mito-TEMPO","entity_type_key":"small_molecule"},"object":{"id":"8a96d7c7-d28d-5615-94a7-3c397bed8f9d","slug":"rac1","display_name":"RAC1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e481a78f-cbe1-5a09-9a04-6d2c1cc651c1","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-mitoros-rescue-event","event_type":"observed_relationship","label":"Two different probes help test the oxidative-stress route.","description":"Mito-TEMPO and NAC prevented tested cucurbitacin I effects on Rac1 activation, supporting mitochondrial ROS involvement alongside the thiol-interception caveat for NAC.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"26bd3a3e-663f-5af9-8427-cf8ddc1f6f25","slug":"mitotempo","display_name":"Mito-TEMPO","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a96d7c7-d28d-5615-94a7-3c397bed8f9d","slug":"rac1","display_name":"RAC1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b5fa7a22-e5a7-58d9-9e39-91a611aa431e","slug":"n-acetylcysteine","display_name":"N-Acetyl-L-cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human breast-cancer pharmacology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Probe rescue does not identify the ROS-generating enzyme or prove the same route after human ingestion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Two different probes help test the oxidative-stress route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin I inhibits Rac1 activation in breast cancer cells by a reactive oxygen species-mediated mechanism and independently of Janus tyrosine kinase 2 and P-Rex1. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23478800/ · DOI 10.1124/mol.112.084293","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"306830d3-e46c-53cd-8041-73862fff8dc5","evidence_kind":"source_excerpt","locator":"Lines 180-186","start_line":180,"end_line":186,"excerpt":"## cucurbitacin-i-mitoros-rescue\nTwo different probes help test the oxidative-stress route.\nMito-TEMPO and NAC prevented tested cucurbitacin I effects on Rac1 activation, supporting mitochondrial ROS involvement alongside the thiol-interception caveat for NAC.\nModel: Human breast-cancer pharmacology.\nLimitations: Probe rescue does not identify the ROS-generating enzyme or prove the same route after human ingestion.\nEvidence access: Primary abstract\nCucurbitacin I inhibits Rac1 activation in breast cancer cells by a reactive oxygen species-mediated mechanism and independently of Janus tyrosine kinase 2 and P-Rex1. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23478800/ · DOI 10.1124/mol.112.084293","model_system":"Human breast-cancer pharmacology.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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