{"id":"bacc2698-09af-5d74-b4d3-2d9d3974ebe9","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:membrane-leakage-null","predicate":"no_detected_change_in_recorded_experiment","statement":"Allicin did not produce detected leakage, fusion or aggregation in the tested membrane assays.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"e4d65fb5-12da-57cb-9e67-bf9e86dbb77b","mechanism_event_label":"Allicin did not produce detected leakage, fusion or aggregation in the tested membrane assays.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"c24c2cf1-f8de-502b-b17b-09b09ddf2e0a","slug":"allicin-membrane-leakage-assay","display_name":"Leakage in allicin-exposed membrane assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e4d65fb5-12da-57cb-9e67-bf9e86dbb77b","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:membrane-leakage-null-event","event_type":"experimental_observation","label":"Allicin did not produce detected leakage, fusion or aggregation in the tested membrane assays.","description":"**Membrane entry.** Allicin crossed phospholipid vesicles and human erythrocyte membranes and reacted with enclosed thiols. The investigators did not find membrane leakage, fusion, or aggregation in those assays. Permeation supports intracellular target access during direct exposure; it does not establish oral delivery to a distant tissue or make transport and resistance irrelevant in every organism. [Miron 2000](https://pubmed.ncbi.nlm.nih.gov/10631291/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Allicin exposure","sequence_order":0,"notes":""},{"entity":{"id":"c24c2cf1-f8de-502b-b17b-09b09ddf2e0a","slug":"allicin-membrane-leakage-assay","display_name":"Leakage in allicin-exposed membrane assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Allicin exposure\", \"comparator\": \"Corresponding membrane assay control\", \"endpoint\": \"Allicin did not produce detected leakage, fusion or aggregation in the tested membrane assays.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Artificial membrane assays; primary abstract; exact exposure unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not proof of safety of all membranes, doses, or tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Allicin did not produce detected leakage, fusion or aggregation in the tested membrane assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. | 2000 | DOI 10.1016/s0005-2736(99)00174-1 | PMID 10631291 | https://pubmed.ncbi.nlm.nih.gov/10631291/ | https://doi.org/10.1016/s0005-2736(99)00174-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 27-27; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0dc66864-7573-50e1-9f09-800b411235c3","evidence_kind":"source_excerpt","locator":"Lines 27-27","start_line":27,"end_line":27,"excerpt":"**Membrane entry.** Allicin crossed phospholipid vesicles and human erythrocyte membranes and reacted with enclosed thiols. The investigators did not find membrane leakage, fusion, or aggregation in those assays. Permeation supports intracellular target access during direct exposure; it does not establish oral delivery to a distant tissue or make transport and resistance irrelevant in every organism. [Miron 2000](https://pubmed.ncbi.nlm.nih.gov/10631291/)","model_system":"Artificial membrane assays; primary abstract; exact exposure unextracted.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}