{"id":"ba9d2da2-12e3-5a1d-8ecd-f316d466ffad","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-egte-plp","predicate":"supports_catalysis","statement":"Purified Mycobacterium smegmatis EgtE catalyzed a PLP-dependent C-S lyase reaction in ergothioneine biosynthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"52ebd607-bddc-5c64-8dac-a4cfd168b313","mechanism_event_label":"The active vitamin B6 cofactor participates in microbial synthesis.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"dc1ac837-e506-5809-9dfe-21aece031fcf","slug":"mycobacterium-smegmatis-egte","display_name":"Mycobacterium smegmatis EgtE","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"52ebd607-bddc-5c64-8dac-a4cfd168b313","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-egte-plp-event","event_type":"observed_relationship","label":"The active vitamin B6 cofactor participates in microbial synthesis.","description":"Purified Mycobacterium smegmatis EgtE catalyzed a PLP-dependent C-S lyase reaction in ergothioneine biosynthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dc1ac837-e506-5809-9dfe-21aece031fcf","slug":"mycobacterium-smegmatis-egte","display_name":"Mycobacterium smegmatis EgtE","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f7086495-5e10-536b-81b3-e63590cf156d","slug":"hercynylcysteine-sulfoxide","display_name":"Hercynylcysteine sulfoxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial EgtE with thioether/sulfoxide substrates and reductant comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No demonstrated B6-dependent synthesis in human cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The active vitamin B6 cofactor participates in microbial synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis: the involvement of a sulfenic acid intermediate. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26149121/ · DOI 10.1038/srep11870","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4a598dd4-5407-5267-8ee4-4f4078b4f321","evidence_kind":"source_excerpt","locator":"Lines 200-206","start_line":200,"end_line":206,"excerpt":"## ergothioneine-egte-plp\nThe active vitamin B6 cofactor participates in microbial synthesis.\nPurified Mycobacterium smegmatis EgtE catalyzed a PLP-dependent C-S lyase reaction in ergothioneine biosynthesis.\nModel: Purified bacterial EgtE with thioether/sulfoxide substrates and reductant comparisons.\nLimitations: No demonstrated B6-dependent synthesis in human cells.\nEvidence access: Primary abstract\nMechanistic studies of a novel C-S lyase in ergothioneine biosynthesis: the involvement of a sulfenic acid intermediate. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26149121/ · DOI 10.1038/srep11870","model_system":"Purified bacterial EgtE with thioether/sulfoxide substrates and reductant comparisons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}