{"id":"ba774ef2-52a8-5c24-8056-e1202f6dd5da","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-colon-redox","predicate":"changes_tested","statement":"The study reported an improved glutathione-related redox ratio with SAC during PhIP exposure.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bce3c8bc-ada9-52dd-8624-ed2412d4056c","mechanism_event_label":"The glutathione readout is retained without inventing a precursor flux.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"e11cf305-96be-5490-b804-1a96f0d3eb81","slug":"human-sac-colon-glutathione-redox","display_name":"Glutathione redox readout in SAC-treated human colonic cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"bce3c8bc-ada9-52dd-8624-ed2412d4056c","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-colon-redox-event","event_type":"observed_relationship","label":"The glutathione readout is retained without inventing a precursor flux.","description":"The study reported an improved glutathione-related redox ratio with SAC during PhIP exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e11cf305-96be-5490-b804-1a96f0d3eb81","slug":"human-sac-colon-glutathione-redox","display_name":"Glutathione redox readout in SAC-treated human colonic cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC with PhIP challenge and corresponding cell controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Normal human colonic mucosal epithelial cells exposed to PhIP","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract describes the ratio ambiguously as GSH to GSH/GSSG; exact numerator/denominator require full-text clarification. No cysteine donation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The glutathione readout is retained without inventing a precursor flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[35753083] S-Allylcysteine Potently Protects against PhIP-Induced DNA Damage via Nrf2/AhR Signaling Pathway Modulation in Normal Human Colonic Mucosal Epithelial Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35753083/ · DOI 10.1002/mnfr.202101141","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Normal human colonic mucosal epithelial cells exposed to PhIP","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"27dbb0a7-40d4-5272-b0cf-62f0953276a8","evidence_kind":"source_excerpt","locator":"Lines 176-183","start_line":176,"end_line":183,"excerpt":"## s-allylcysteine-colon-redox\nThe glutathione readout is retained without inventing a precursor flux.\nThe study reported an improved glutathione-related redox ratio with SAC during PhIP exposure.\nModel: Normal human colonic mucosal epithelial cells exposed to PhIP\nLimitations: The abstract describes the ratio ambiguously as GSH to GSH/GSSG; exact numerator/denominator require full-text clarification. No cysteine donation is inferred.\nEvidence access: Primary abstract\n[35753083] S-Allylcysteine Potently Protects against PhIP-Induced DNA Damage via Nrf2/AhR Signaling Pathway Modulation in Normal Human Colonic Mucosal Epithelial Cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35753083/ · DOI 10.1002/mnfr.202101141\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"Normal human colonic mucosal epithelial cells exposed to PhIP\", \"dose\": \"Not specified in accessed abstract\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"Cell culture\", \"experimental_comparison\": \"SAC with PhIP challenge and corresponding cell controls\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Normal human colonic mucosal epithelial cells exposed to PhIP","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"91d7aeac82e42ce559d13dd8a2e0165d37760f7f555dd01c80e0604fa6e71ea2","revision_id":"545e497c-e5e3-543c-b26b-b297cbc7bbec","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}