{"id":"ba5cf09f-5867-530f-b70f-ae3bd4e4b7e0","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ferroptosis-gsh-null","predicate":"does_not_deplete","statement":"Polyamine treatment did not deplete cellular glutathione in the reported comparison.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"682e52e3-de4e-5f8e-9d58-281e2399436d","mechanism_event_label":"Iron-dependent injury did not require measured glutathione depletion.","subject":{"id":"827038ae-56e6-5047-aea1-3010ff8d8a12","slug":"polyamine-treatment-in-ferroptosis-study","display_name":"Polyamine treatment in the A549/HT1080 ferroptosis study","entity_type_key":"cellular_process"},"object":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"682e52e3-de4e-5f8e-9d58-281e2399436d","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-ferroptosis-gsh-null-event","event_type":"observed_relationship","label":"Iron-dependent injury did not require measured glutathione depletion.","description":"Polyamine treatment did not deplete cellular glutathione in the reported comparison.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"827038ae-56e6-5047-aea1-3010ff8d8a12","slug":"polyamine-treatment-in-ferroptosis-study","display_name":"Polyamine treatment in the A549/HT1080 ferroptosis study","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human A549/HT1080 experiments; polyamine panel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not negate GSH protection or generalize to every dose and cell type.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Iron-dependent injury did not require measured glutathione depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Polyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38504107/ · DOI 10.1038/s41467-024-46776-w","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b5c8e124-88b0-5159-8987-0c7e8b172bd7","evidence_kind":"source_excerpt","locator":"Lines 542-548","start_line":542,"end_line":548,"excerpt":"## spermidine-ferroptosis-gsh-null\nIron-dependent injury did not require measured glutathione depletion.\nPolyamine treatment did not deplete cellular glutathione in the reported comparison.\nModel: Human A549/HT1080 experiments; polyamine panel.\nLimitations: Does not negate GSH protection or generalize to every dose and cell type.\nEvidence access: Primary full text\nPolyamine-mediated ferroptosis amplification acts as a targetable vulnerability in cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38504107/ · DOI 10.1038/s41467-024-46776-w","model_system":"Human A549/HT1080 experiments; polyamine panel.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}