{"id":"ba5cdfa1-057f-52f0-b805-bb27afda6644","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-apt2-binding","predicate":"has_contextual_experimental_effect","statement":"Sulforaphane-affinity beads captured APT2 from Huh-7 or transfected HEK293 lysates; C56S mutation attenuated binding in the reported assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"24044adf-ada9-536d-ad21-2052958954bd","mechanism_event_label":"sfn apt2 binding","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"24044adf-ada9-536d-ad21-2052958954bd","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-apt2-binding","event_type":"experimental_result","label":"sfn apt2 binding","description":"Sulforaphane-affinity beads captured APT2 from Huh-7 or transfected HEK293 lysates; C56S mutation attenuated binding in the reported assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round6-sources/sulforaphane-apt2.pdf; SHA256 31e2993f88fe05eec199b9013173b29379b3346b9696ac766771173ddd0f181a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Huh-7 and HEK293 cell lysates; endogenous or FLAG-tagged human APT2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Bead pulldown; free-sulforaphane competition at 100 micromolar for 30 minutes at 4 C. 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Mutagenesis supports C56-dependent engagement; no binding affinity or substrate-specific catalytic inhibition constant was established.","model_system":"Human Huh-7 and HEK293 cell lysates; endogenous or FLAG-tagged human APT2","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase and primary locator; not a verbatim publisher quote. 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